Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159, R127A, R130A) (Biotin,His-Avi Tag)

Catalog No. ABIN7275808

This Recombinant Thymic Stromal Lymphopoietin protein is expressed in HEK-293 Cells.

Quick Overview for Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159, R127A, R130A) (Biotin,His-Avi Tag) (ABIN7275808)

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-159, R127A, R130A

Purification tag / Conjugate: This Thymic Stromal Lymphopoietin protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human TSLP (R127A, R130A) Protein

Sequence: Tyr29-Gln159(R127A,R130A)

Characteristics: Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.,Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.,Biotinylated Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 4.87 nM as determined in BLI assay (Gator® Prime).,Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Molecular Weight: 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result.

UniProt: Q969D9