ULBP1 Protein (His-Avi Tag)
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- Target See all ULBP1 Proteins
- ULBP1 (UL16 Binding Protein 1 (ULBP1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ULBP1 protein is labelled with His-Avi Tag.
- Sequence
- Gly26-Pro215
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ULBP-1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA. See testing image for detail.
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UL16 Binding Protein 1 (ULBP1) protein (His-Avi Tag,Biotin)
ULBP1 Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
UL16 Binding Protein 1 (ULBP1) (AA 26-216) protein (His tag)ULBP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
UL16 Binding Protein 1 (ULBP1) (AA 1-244) protein (GST tag)ULBP1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
UL16 Binding Protein 1 (ULBP1) (AA 26-216) protein (His tag)Crystallography grade ULBP1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM PB, 0.5M NaCl, 0.1M L-arginine ( pH 8.0). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ULBP1 (UL16 Binding Protein 1 (ULBP1))
- Alternative Name
- ULBP-1 (ULBP1 Products)
- Synonyms
- RAET1I Protein, A430108B07Rik Protein, MULT1 Protein, ULBP1 Protein, UL16 binding protein 1 Protein, UL16 binding protein 21 Protein, NKG2D ligand 1 Protein, ULBP1 Protein, ULBP21 Protein, Ulbp1 Protein, LOC100683099 Protein
- Background
- ALCAN-beta, NKG2D ligand 1, N2DL-1, NKG2DL1, MULT-1, NKG2DL, AET1I, ULBP1, ULBP-1,Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.
- Molecular Weight
- 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9BZM6
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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