VCAM1 Protein (AA 25-697) (His tag)

Catalog No. ABIN7275819

Product Details

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Protein Type: Recombinant

Protein Characteristics: AA 25-697

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This VCAM1 protein is labelled with His tag.

Purpose: Human VCAM-1/CD106 Protein

Sequence: Phe25-Pro697

Characteristics: Recombinant Human VCAM-1/CD106 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Pro697.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: VCAM-1 (VCAM1 Products)

Synonyms: CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein

Background: Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.

Molecular Weight: 75.2 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_001069

Pathways: Carbohydrate Homeostasis