FLT4 Protein (AA 25-776) (His-Avi Tag)

Catalog No. ABIN7275839

Product Details

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Protein Type: Recombinant

Protein Characteristics: AA 25-776

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FLT4 protein is labelled with His-Avi Tag.

Purpose: Human VEGF R3/FLT4 Protein

Sequence: Tyr25-Ile776

Characteristics: Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Alternative Name: VEGF R3 (FLT4 Products)

Synonyms: FLT41 Protein, LMPH1A Protein, PCL Protein, VEGFR3 Protein, AI323512 Protein, Chy Protein, Flt-4 Protein, VEGFR-3 Protein, Vegfr3 Protein, cb1059 Protein, fms related tyrosine kinase 4 Protein, FMS-like tyrosine kinase 4 Protein, fms-related tyrosine kinase 4 Protein, FLT4 Protein, Flt4 Protein, flt4 Protein

Background: Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.

Molecular Weight: 87.4 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling