Aggrecan Protein (ACAN) (AA 17-721) (His tag)

Catalog No. ABIN7454069

Product Details

Target: Aggrecan (ACAN)

Protein Type: Recombinant

Protein Characteristics: AA 17-721

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Aggrecan protein is labelled with His tag.

Purpose: Human Aggrecan Protein

Sequence: Ala17-Leu721

Characteristics: Recombinant Human Aggrecan Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala17-Leu721.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan (ACAN Products)

Synonyms: AGC1 Protein, AGCAN Protein, CSPG1 Protein, CSPGCP Protein, MSK16 Protein, SEDK Protein, Agc Protein, Agc1 Protein, Cspg1 Protein, b2b183Clo Protein, cmd Protein, aggrecan Protein, aggrecan core protein Protein, ACAN Protein, Acan Protein, LOC100380783 Protein, AGC Protein

Background: Aggrecan is a large proteoglycan that forms giant hydrated aggregates with hyaluronan in the extracellular matrix (ECM). The extraordinary resistance of these aggregates to compression explains their abundance in articular cartilage of joints where they ensure adequate load-bearing. In the brain, they provide mechanical buffering and contribute to formation of perineuronal nets, which regulate synaptic plasticity. Aggrecan is also present in cardiac jelly, developing heart valves, and blood vessels during cardiovascular development. Whereas aggrecan is essential for skeletal development, its function in the developing cardiovascular system remains to be fully elucidated.

Molecular Weight: 78.86 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.

UniProt: Q6PID9

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport