CD44 Protein (CD44) (AA 21-220) (His tag)

Catalog No. ABIN7454096

Product Details

Target: CD44

Protein Type: Recombinant

Protein Characteristics: AA 21-220

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD44 protein is labelled with His tag.

Purpose: Human CD44 Protein

Sequence: Gln21-Pro220 & Gln386-Ala427

Characteristics: Recombinant Human CD44 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Pro220 & Gln386-Ala427.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD44, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD44

Alternative Name: CD44 (CD44 Products)

Synonyms: CDW44 Protein, CSPG8 Protein, ECMR-III Protein, HCELL Protein, HUTCH-I Protein, IN Protein, LHR Protein, MC56 Protein, MDU2 Protein, MDU3 Protein, MIC4 Protein, Pgp1 Protein, AU023126 Protein, AW121933 Protein, AW146109 Protein, HERMES Protein, Ly-24 Protein, Pgp-1 Protein, CD44A Protein, METAA Protein, RHAMM Protein, XCD44 Protein, cd44 Protein, cdw44 Protein, cspg8 Protein, ecmr-iii Protein, hcell Protein, lhr Protein, mc56 Protein, mdu2 Protein, mdu3 Protein, mic4 Protein, mutch-i Protein, pgp1 Protein, CD44 molecule (Indian blood group) Protein, syndecan 4 Protein, CD44 antigen Protein, CD44 molecule (Indian blood group) S homeolog Protein, CD44 Protein, SDC4 Protein, Cd44 Protein, cd44.S Protein

Background: CD44 is a hyaluronan binding cell surface signal transducing receptor that influences motility, cell survival and proliferation as well as the formation of tumor microenvironment. CD44 contains two variable regions encoded by variable exons. Alternative splicing, which is often deregulated in cancer, can produce various isoforms of CD44 with properties that may have different tissue specific effects and therefore even diverse effects on cancer progression

Molecular Weight: 27.91 kDa. Due to glycosylation, the protein migrates to 45-68 kDa based on Tris-Bis PAGE result.

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling