R-Spondin 3 Protein (RSPO3) (AA 22-146) (His tag)
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- Target See all R-Spondin 3 (RSPO3) Proteins
- R-Spondin 3 (RSPO3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-146
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This R-Spondin 3 protein is labelled with His tag.
- Purpose
- Human R spondin 3/RSPO3 Protein
- Sequence
- Gln22-Val146
- Characteristics
- Recombinant Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val146.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product RSPO3 Protein
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R-Spondin 3 (RSPO3) (AA 24-272) protein (His-IF2DI Tag)
RSPO3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
R-Spondin 3 (RSPO3) (AA 22-272) protein (His tag)Crystallography grade RSPO3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
R-Spondin 3 (RSPO3) (AA 22-277) protein (His tag)Crystallography grade RSPO3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- R-Spondin 3 (RSPO3)
- Alternative Name
- R spondin 3 (RSPO3 Products)
- Synonyms
- pwtsr Protein, thsd2 Protein, cristin1 Protein, CRISTIN1 Protein, PWTSR Protein, THSD2 Protein, RGD1563246 Protein, 2810459H04Rik Protein, AW742308 Protein, Cristin1 Protein, Thsd2 Protein, R-spondin 3 Protein, rspo3 Protein, RSPO3 Protein, Rspo3 Protein
- Background
- The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
- Molecular Weight
- 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
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