HLA-A*24:02 (Monomer) protein (MAGE-A3)

Catalog No. ABIN7454319

Product Details

Target: HLA-A*24:02

Protein Type: Recombinant

Protein Characteristics: Monomer

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: MAGE-A3

Purpose: Human HLA-A*24:02 & B2M & MAGE-A3 (IMPKAGLLI) Monomer Protein

Sequence: Gly25-Thr305 (HLA-A*24:02), Ile21-Met119 (B2M) and IMPKAGLLI peptide

Specificity: Uni-Prot: AAA59600.1 (HLA-A*24:02), P61769 (B2M), IMPKAGLLI

Characteristics: Recombinant Human MAGE-A3(HLA-A*24:02) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and IMPKAGLLI peptide.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLA-A*24:02

Background: Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.

Molecular Weight: 50.20 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.