CTXB Protein (His tag)

Catalog No. ABIN7482638

This Recombinant CTXB protein is produced in HEK-293 Cells.

Quick Overview for CTXB Protein (His tag) (ABIN7482638)

Target: CTXB (CtxB Gene Product (CTXB))

Protein Type: Recombinant

Origin: Vibrio sp.

Source: HEK-293 Cells

Purity: 95 %

Grade: MALS verified

Product Details

Purification tag / Conjugate: This CTXB protein is labelled with His tag.

Purpose: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Cholera enterotoxin subunit B Protein, His Tag (MALS verified)

Sequence: Thr 22 - Asn 124

Characteristics: Vibrio cholerae serotype O1 Cholera enterotoxin subunit B, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Asn 124 (Accession # P01556).

Endotoxin Level: 1.0 EU per μg

Application Details

Comment:

This protein carries a polyhistidine tag at the C-terminus. (10xHis) The protein has a calculated MW of 13.5 kDa. The protein migrates as 16-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: CTXB (CtxB Gene Product (CTXB))

Alternative Name: Cholera Enterotoxin Subunit B

Background: Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge.

Molecular Weight: 13.5 kDa