Prothrombin Protein (AA 25-617) (His tag)
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- Target See all Prothrombin (F2) Proteins
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-617
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Origin
- Rat
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Prothrombin protein is labelled with His tag.
- Purpose
- Rat Coagulation Factor II Protein
- Sequence
- Gln25-Arg617
- Characteristics
- Recombinant Rat Coagulation Factor II Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Arg617.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Coagulation Factor II (thrombin) (F2) (AA 364-622) protein (His tag)Crystallography grade F2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Coagulation Factor II (thrombin) (F2) (AA 361-618) protein (His tag)Crystallography grade F2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Coagulation Factor II (thrombin) (F2) (AA 44-622) protein (His tag)Crystallography grade F2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Coagulation Factor II (thrombin) (F2) (AA 44-618) protein (His tag)Crystallography grade F2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- Alternative Name
- Coagulation Factor II (F2 Products)
- Synonyms
- PT Protein, RPRGL2 Protein, THPH1 Protein, Cf-2 Protein, Cf2 Protein, FII Protein, thrombin Protein, zgc:66319 Protein, wu:fb57c10 Protein, wu:fd42e09 Protein, wu:fd59d01 Protein, prothrombin Protein, F2 Protein, coagulation factor II, thrombin Protein, coagulation factor II Protein, coagulation factor II (thrombin) Protein, F2 Protein, f2 Protein
- Background
- Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites.
- Molecular Weight
- 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_075213
- Pathways
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
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