EPO Protein (AA 28-193)

Catalog No. ABIN7504320

This Recombinant EPO protein is produced in HEK-293 Cells.

Quick Overview for EPO Protein (AA 28-193) (ABIN7504320)

Target: EPO (Erythropoietin (EPO))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-193

Purpose: Human EPO/Erythropoietin Protein

Sequence: Ala28-Arg193

Characteristics: Recombinant Human EPO/Erythropoietin Protein is expressed from HEK293 without tag.It contains Ala28-Arg193.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human EPO, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 46.1ng/ml determined by ELISA.,Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50-for this effect is 0.4 - 1 ng/mL (QC Test).,The purity of Human EPO is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Background: Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects.

Molecular Weight: 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity