PBK Protein (AA 1-322) (His tag)
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- Target See all PBK Proteins
- PBK (PDZ Binding Kinase (PBK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-322
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PBK protein is labelled with His tag.
- Purpose
- Human PBK/TOPK Protein
- Sequence
- Met1-Val322
- Characteristics
- Recombinant Human PBK/TOPK Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met1-Val322.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product PBK Protein
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PDZ Binding Kinase (PBK) (AA 1-322) (Active) protein (GST tag)
PBK Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
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PDZ Binding Kinase (PBK) (AA 1-322) protein (His tag)PBK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
PDZ Binding Kinase (PBK) (AA 1-322) protein (GST tag)PBK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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PDZ Binding Kinase (PBK) (AA 122-229) protein (GST tag)PBK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
PDZ Binding Kinase (PBK) (AA 1-330) protein (Strep Tag)Crystallography grade PBK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
PDZ Binding Kinase (PBK) protein (Myc-DYKDDDDK Tag)PBK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PBK (PDZ Binding Kinase (PBK))
- Alternative Name
- PBK (PBK Products)
- Synonyms
- PBK Protein, topk Protein, CT84 Protein, Nori-3 Protein, SPK Protein, TOPK Protein, 2810434B10Rik Protein, AW538537 Protein, D14Ertd732e Protein, zgc:92050 Protein, PDZ binding kinase Protein, PDZ binding kinase L homeolog Protein, PBK Protein, pbk Protein, Pbk Protein, pbk.L Protein
- Background
- T-lymphokine-activated killer cell-originated protein kinase (TOPK, also known as PDZ-binding kinase or PBK) plays a crucial role in cell cycle regulation and mitotic progression. Abnormal overexpression or activation of TOPK has been observed in many cancers, including colorectal cancer, triple-negative breast cancer, and melanoma, and it is associated with increased development, dissemination, and poor clinical outcomes and prognosis in cancer. Moreover, TOPK phosphorylates p38, JNK, ERK, and AKT, which are involved in many cellular functions, and participates in the activation of multiple signaling pathways related to MAPK, PI3K/PTEN/AKT, and NOTCH1.
- Molecular Weight
- 37.61 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
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