RSV Fusion Protein Protein (His tag)
RSV F
Origin: Respiratory Syncytial Virus (RSV)
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
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- Target See all RSV Fusion Protein (RSV F) products
- RSV Fusion Protein (RSV F) (Respiratory Syncytial Virus Fusion Protein (RSV F))
- Protein Type
- Recombinant
- Origin
- Respiratory Syncytial Virus (RSV)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RSV Fusion Protein protein is labelled with His tag.
- Purpose
- RSV (A) Pre-fusion glycoprotein F0 Protein
- Characteristics
- Recombinant RSV (A) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Respiratory Syncytial Virus Fusion Protein (RSV F) protein (His tag)
RSV F Origin: Respiratory Syncytial Virus (RSV) Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- RSV Fusion Protein (RSV F) (Respiratory Syncytial Virus Fusion Protein (RSV F))
- Alternative Name
- RSV Pre-fusion glycoprotein F0 (RSV F Products)
- Background
- Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site which serves as a primary target of neutralizing antibodies in the body.
- Molecular Weight
- 56.28 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Tris-Bis PAGE result.
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