CD247 Protein (AA 52-164) (His-SUMO Tag)

Catalog No. ABIN7504947

Product Details

Target: CD247 (CD247 Molecule (CD247))

Protein Type: Recombinant

Protein Characteristics: AA 52-164

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CD247 protein is labelled with His-SUMO Tag.

Sequence: Arg52-Arg164

Characteristics: A DNA sequence encoding the Human CD247/CD3-ZETA protein (P20963) (Arg52-Arg164) was expressed with a N-His-SUMO tag.

Purity: >85 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris-HCl, 150 mM NaCI, 1 mM DTT ( pH 8.0), Mannitol, Proclin300, Tween 80

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD247 (CD247 Products)

Synonyms: CD3-ZETA Protein, CD3H Protein, CD3Q Protein, CD3Z Protein, T3Z Protein, TCRZ Protein, 4930549J05Rik Protein, A430104F18Rik Protein, AW552088 Protein, Cd3 Protein, Cd3-eta Protein, Cd3-zeta Protein, Cd3h Protein, Cd3z Protein, Cd3zeta Protein, T3z Protein, Tcrk Protein, Tcrz Protein, LOC100136514 Protein, LOC100136549 Protein, LOC100305023 Protein, CD247 Protein, TCRzeta Protein, CD247 molecule Protein, CD247 antigen Protein, CD3zeta-2 Protein, CD3zeta-1 Protein, Cd247 molecule Protein, CD247 Protein, Cd247 Protein, cd3z Protein, cd247 Protein

Background:

Background: CD247, also known as CD3-ZETA, belongs to the CD3Z/FCER1G family. It contains 3 ITAM domains. As a -cell receptor zeta, CD247 forms the T-cell receptor-CD3 complex together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and –epsilon. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. Defects in CD247 can cause immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

Synonym: CD3-ZETA;CD3H;CD3Q;CD3Z;IMD25;T3Z;TCRZ

Molecular Weight: 13 kDa

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway