Cathepsin S Protein (CTSS) (His tag)

Catalog No. ABIN7519746

Product Details

Target: Cathepsin S (CTSS)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Cathepsin S protein is labelled with His tag.

Purpose: Active Recombinant Mouse Cathepsin S/CTSS Protein

Sequence: VCSVAMEQLQ RDPTLDYHWD LWKKTHEKEY KDKNEEEVRR LIWEKNLKFI MIHNLEYSMG MHTYQVGMND MGDMTNEEIL CRMGALRIPR QSPKTVTFRS YSNRTLPDTV DWREKGCVTE VKYQGSCGAC WAFSAVGALE GQLKLKTGKL ISLSAQNLVD CSNEEKYGNK GCGGGYMTEA FQYIIDNGGI EADASYPYKA TDEKCHYNSK NRAATCSRYI QLPFGDEDAL KEAVATKGPV SVGIDASHSS FFFYKSGVYD DPSCTGNVNH GVLVVGYGTL DGKDYWLVKN SWGLNFGDQG YIRMARNNKN HCGIASYCSY PEI

Specificity: Val18-Ile340

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE- Nval-WRK(Dnp)-NH2 . The specific activity is >212 pmol/ min/μg, as measured under the described conditions.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: Cathepsin S (CTSS)

Alternative Name: Cathepsin S/CTSS (CTSS Products)

Synonyms: ctss-a Protein, MGC69026 Protein, ctss-b Protein, MGC80097 Protein, ctss.1 Protein, CTSS Protein, ctss Protein, cats Protein, cathepsin S Protein, cathepsin S L homeolog Protein, cathepsin S S homeolog Protein, Cathepsin S Protein, CTSS Protein, Ctss Protein, ctss.L Protein, ctss.S Protein, ctss Protein, cats Protein

Background:

Description: Cathepsin S (CTSS), one of the lysosomal proteinases, has many important physiological functions in the nervous system, especially in process of extracellular matrix degradation and endocellular antigen presentation. Cathepsin S is expressed in the lysosome of antigen presenting cells, primarily dendritic cells, B-cells and macrophages. Cathepsin S is most well known for its critical function in the proteolytic digestion of the invariant chain chaperone molecules, thus controlling antigen presentation to CD4+ T-cells by major histocompatibility complex (MHC) class II molecules or to NK1.1+ T-cells via CD1 Molecules. Cathepsin S also appears to participate in direct processing of exogenous antigens for presentation by MHC class II to CD4+ T-cells, or in cross-presentation by MHC class I molecules to CD8+ T-cells. In addition, it has been implicated in the pathogenesis of several diseases such as Alzheimer's disease and degenerative disorders associated with the cells of the mononuclear phagocytic system.

Name: Cathepsin S, Ctss, Cats,CTSS

Gene ID: 13040

UniProt: O70370

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades