CER1 Protein (His tag)

Catalog No. ABIN7536458

Product Details

Target: CER1 (Cerberus 1 Homolog (Xenopus Laevis) (CER1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CER1 protein is labelled with His tag.

Purpose: Recombinant Human CER1/DAND4 Protein

Sequence: TRHQDGRQNQ SSLSPVLLPR NQRELPTGNH EEAEEKPDLF VAVPHLVATS PAGEGQRQRE KMLSRFGRFW KKPEREMHPS RDSDSEPFPP GTQSLIQPID GMKMEKSPLR EEAKKFWHHF MFRKTPASQG VILPIKSHEV HWETCRTVPF SQTITHEGCE KVVVQNNLCF GKCGSVHFPG AAQHSHTSCS HCLPAKFTTM HLPLNCTELS SVIKVVMLVE ECQCKVKTEH EDGHILHAGS QDSFIPGVSA

Specificity: Thr18-Ala267

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.01EU/μg

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 0.42 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of 25 mM NaAc pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: CER1 (Cerberus 1 Homolog (Xenopus Laevis) (CER1))

Alternative Name: CER1/DAND4 (CER1 Products)

Synonyms: DAND4 Protein, CAR Protein, cer Protein, cer-1 Protein, dand4 Protein, tCerberus Protein, xcer-1 Protein, xcer1 Protein, Cerl Protein, Cerl1 Protein, Cerr1 Protein, RGD1563046 Protein, cerberus 1, DAN family BMP antagonist Protein, cerberus 1, DAN family BMP antagonist S homeolog Protein, CER1 Protein, cer1.S Protein, Cer1 Protein

Background:

Description: Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus, DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands. Mature human Cerberus 1 shares 67 % and 68 % amino acid (aa) sequence identity with mouse and rat Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24 %-37 % aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms homodimers. Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain. At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh . Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation. In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction.

Name: DAND4,CER1

Gene ID: 9350

UniProt: O95813

Pathways: Maintenance of Protein Location