CD55 Protein (His tag)

Catalog No. ABIN7534557

Product Details

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD55 protein is labelled with His tag.

Purpose: Active Recombinant Human DAF/CD55 Protein

Sequence: DCGLPPDVPN AQPALEGRTS FPEDTVITYK CEESFVKIPG EKDSVICLKG SQWSDIEEFC NRSCEVPTRL NSASLKQPYI TQNYFPVGTV VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREIY CPAPPQIDNG IIQGERDHYG YRQSVTYACN KGFTMIGEHS IYCTVNNDEG EWSGPPPECR GKSLTSKVPP TVQKPTTVNV PTTEVSPTSQ KTTTKTTTPN AQATRSTPVS RTTKHFHETT PNKGSGTTS

Specificity: Asp35-Ser353

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Human DAF/CD55 at 0.5 μg/mL (100 μL/well) can bind Human CD97 with a linear range of 0.3-23.8 ng/mL.|2.Measured by its binding ability in a functional ELISA. Immobilized APC anti-human CD55 Antibody at 1 μg/mL (25 μL/well) can bind Recombinant Human CD55 with a linear range of 0.5-3.2 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: DAF/CD55 (CD55 Products)

Synonyms: CR Protein, CROM Protein, DAF Protein, TC Protein, Daf Protein, Daf-GPI Protein, Daf1 Protein, GPI-DAF Protein, CD55 molecule (Cromer blood group) Protein, CD55 molecule, decay accelerating factor for complement Protein, CD55 Protein, Cd55 Protein

Background:

Description: This protein is a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM).

Name: CD55,CR,CROM,DAF,TC, CROM, DAF, TC

Gene ID: 1604

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity