Decorin Protein (DCN) (His tag)

Catalog No. ABIN7535247

Product Details

Target: Decorin (DCN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Decorin protein is labelled with His tag.

Purpose: Active Recombinant Human Decorin/PG-S2/DCN Protein

Sequence: GPFQQRGLFD FMLEDEASGI GPEVPDDRDF EPSLGPVCPF RCQCHLRVVQ CSDLGLDKVP KDLPPDTTLL DLQNNKITEI KDGDFKNLKN LHALILVNNK ISKVSPGAFT PLVKLERLYL SKNQLKELPE KMPKTLQELR AHENEITKVR KVTFNGLNQM IVIELGTNPL KSSGIENGAF QGMKKLSYIR IADTNITSIP QGLPPSLTEL HLDGNKISRV DAASLKGLNN LAKLGLSFNS ISAVDNGSLA NTPHLRELHL DNNKLTRVPG GLAEHKYIQV VYLHNNNISV VGSSDFCPPG HNTKKASYSG VSLFSNPVQY WEIQPSTFRC VYVRSAIQLG NYK

Specificity: Gly17-Lys359

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human DCN at 1 μg/mL (100 μL/well) can bind DCN Rabbit pAb with a linear range of 0.5-141 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: Decorin (DCN)

Alternative Name: Decorin/PG-S2/DCN (DCN Products)

Synonyms: CSCD Protein, DSPG2 Protein, PG40 Protein, PGII Protein, PGS2 Protein, SLRR1B Protein, DC Protein, mDcn Protein, cscd Protein, dspg2 Protein, pg40 Protein, pgii Protein, pgs2 Protein, slrr1b Protein, decorin Protein, decorin L homeolog Protein, DCN Protein, Dcn Protein, dcn.L Protein, dcn Protein

Background:

Description: Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS). As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. Decorin resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival. Decorin binds to and modulates the signaling of the epidermal growth factor receptor and other members of the ErbB family of receptor tyrosine kinases. It exerts its antitumor activity by a dual mechanism: via inhibition of these key receptors through their physical downregulation coupled with attenuation of their signaling, and by binding to and sequestering TGFbeta. Decorin also modulates the insulin-like growth factor receptor and the low-density lipoprotein receptor-related protein 1, which indirectly affects the TGFbeta receptor pathway. Decorin plays significant roles in tissue development and assembly, as well as playing both direct and indirect signaling roles.

Name: DCN,CSCD,DSPG2,PG40,PGII,PGS2,SLRR1B,decorin,Decorin

Gene ID: 1634

UniProt: P07585-1

Pathways: Glycosaminoglycan Metabolic Process