P-Selectin Protein (Fc Tag,His tag)

Catalog No. ABIN7535437

Product Details

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This P-Selectin protein is labelled with Fc Tag,His tag.

Purpose: Active Recombinant Human SELP/P-Selectin/CD62P Protein

Sequence: WTYHYSTKAY SWNISRKYCQ NRYTDLVAIQ NKNEIDYLNK VLPYYSSYYW IGIRKNNKTW TWVGTKKALT NEAENWADNE PNNKRNNEDC VEIYIKSPSA PGKWNDEHCL KKKHALCYTA SCQDMSCSKQ GECLETIGNY TCSCYPGFYG PECEYVRECG ELELPQHVLM NCSHPLGNFS FNSQCSFHCT DGYQVNGPSK LECLASGIWT NKPPQCLAAQ CPPLKIPERG NMTCLHSAKA FQHQSSCSFS CEEGFALVGP EVVQCTASGV WTAPAPVCKA VQCQHLEAPS EGTMDCVHPL TAFAYGSSCK FECQPGYRVR GLDMLRCIDS GHWSAPLPTC EAISCEPLES PVHGSMDCSP SLRAFQYDTN CSFRCAEGFM LRGADIVRCD NLGQWTAPAP VCQALQCQDL PVPNEARVNC SHPFGAFRYQ SVCSFTCNEG LLLVGASVLQ CLATGNWNSV PPECQAIPCT PLLSPQNGTM TCVQPLGSSS YKSTCQFICD EGYSLSGPER LDCTRSGRWT DSPPMCEAIK CPELFAPEQG SLDCSDTRGE FNVGSTCHFS CDNGFKLEGP NNVECTTSGR WSATPPTCKG IASLPTPGLQ CPALTTPGQG TMYCRHHPGT FGFNTTCYFG CNAGFTLIGD STLSCRPSGQ WTAVTPACRA VKCSELHVNK PIAMNCSNLW GNFSYGSICS FHCLEGQLLN GSAQTACQEN GHWSTTVPTC QAGPLTIQEA

Specificity: Trp42-Ala771

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: <1EU/μg

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to Human SELP coated plates (10 μg/mL with 100 μL/well), > 80% cells will adhere after 1 hour at 37°C.|2.Measured by its binding ability in a functional ELISA.Immobilized PE Mouse Anti-Human CD62P Antibody at 1μg/mL (25 μL/well) can bind Human CD62P with a linear range of 0.46-7.82 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: SELP/P-Selectin/CD62P (SELP Products)

Synonyms: PSELECT Protein, CD62 Protein, CD62P Protein, GMP140 Protein, GRMP Protein, LECAM3 Protein, PADGEM Protein, PSEL Protein, GMP-140 Protein, Grmp Protein, selectin P Protein, selectin, platelet Protein, Selp Protein, SELP Protein

Background:

Description: P selectin (SELP) is a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.

Name: CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL,CD62P/P-selectin,SELP,CD62P,GMP140,GRMP,LECAM3,PADGEM,PSEL

Gene ID: 6403

UniProt: P16109