SPOCK1 Protein (His tag)

Catalog No. ABIN7520523

Recombinant SPOCK1 protein expressed in HEK-293 Cells.

Quick Overview for SPOCK1 Protein (His tag) (ABIN7520523)

Target: SPOCK1 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This SPOCK1 protein is labelled with His tag.

Purpose: Active Recombinant Human Testican-1/SPOCK1 Protein

Sequence: RHLDALAGGA GPNHGNFLDN DQWLSTVSQY DRDKYWNRFR DDDYFRNWNP NKPFDQALDP SKDPCLKVKC SPHKVCVTQD YQTALCVSRK HLLPRQKKGN VAQKHWVGPS NLVKCKPCPV AQSAMVCGSD GHSYTSKCKL EFHACSTGKS LATLCDGPCP CLPEPEPPKH KAERSACTDK ELRNLASRLK DWFGALHEDA NRVIKPTSSN TAQGRFDTSI LPICKDSLGW MFNKLDMNYD LLLDPSEINA IYLDKYEPCI KPLFNSCDSF KDGKLSNNEW CYCFQKPGGL PCQNEMNRIQ KLSKGKSLLG AFIPRCNEEG YYKATQCHGS TGQCWCVDKY GNELAGSRKQ GAVSCEEEQE TSGDFGSGGS VVLLDDLEYE RELGPKDKEG KLRVHTRAVT EDDEDEDDDK EDEVGYIW

Specificity: Arg22-Trp439

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value approximately is 1.7 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: SPOCK1 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1))

Alternative Name: Testican-1/SPOCK1

Background: SPOCK,TIC1,TICN1,Testican-1,SPOCK1

Gene ID: 6695

UniProt: Q08629