SPOCK1 Protein (His tag)
-
- Target See all SPOCK1 Proteins
- SPOCK1 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SPOCK1 protein is labelled with His tag.
- Purpose
- Active Recombinant Human Testican-1/SPOCK1 Protein
- Sequence
- RHLDALAGGA GPNHGNFLDN DQWLSTVSQY DRDKYWNRFR DDDYFRNWNP NKPFDQALDP SKDPCLKVKC SPHKVCVTQD YQTALCVSRK HLLPRQKKGN VAQKHWVGPS NLVKCKPCPV AQSAMVCGSD GHSYTSKCKL EFHACSTGKS LATLCDGPCP CLPEPEPPKH KAERSACTDK ELRNLASRLK DWFGALHEDA NRVIKPTSSN TAQGRFDTSI LPICKDSLGW MFNKLDMNYD LLLDPSEINA IYLDKYEPCI KPLFNSCDSF KDGKLSNNEW CYCFQKPGGL PCQNEMNRIQ KLSKGKSLLG AFIPRCNEEG YYKATQCHGS TGQCWCVDKY GNELAGSRKQ GAVSCEEEQE TSGDFGSGGS VVLLDDLEYE RELGPKDKEG KLRVHTRAVT EDDEDEDDDK EDEVGYIW
- Specificity
- Arg22-Trp439
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value approximately is 1.7 nM.
- Top Product
- Discover our top product SPOCK1 Protein
-
Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1) (AA 1-439) protein (GST tag)
SPOCK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1) (AA 22-439) protein (His tag)Crystallography grade SPOCK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1) (AA 22-442) protein (His tag)Crystallography grade SPOCK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
-
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
-
- Target
- SPOCK1 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 1 (SPOCK1))
- Alternative Name
- Testican-1/SPOCK1 (SPOCK1 Products)
- Synonyms
- SPOCK Protein, TESTICAN Protein, TIC1 Protein, Spock Protein, Ticn1 Protein, testican Protein, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 1 Protein, sparc/osteonectin, cwcv and kazal like domains proteoglycan 1 Protein, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 Protein, SPOCK1 Protein, Spock1 Protein
- Background
- SPOCK,TIC1,TICN1,Testican-1,SPOCK1
- Gene ID
- 6695
- UniProt
- Q08629
-
