Cripto-1 Protein (His tag)
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- Target See all Cripto-1 (TDGF1.3) products
- Cripto-1 (TDGF1.3) (Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Cripto-1 protein is labelled with His tag.
- Purpose
- Active Recombinant Human Cripto-1/TDGF1 Protein
- Sequence
- LGHQEFARPS RGYLAFRDDS IWPQEEPAIR PRSSQRVPPM GIQHSKELNR TCCLNGGTCM LGSFCACPPS FYGRNCEHDV RKENCGSVPH DTWLPKKCSL CKCWHGQLRC FPQAFLPGCD GLVMDEHLVA SRTPELPPSA RT
- Specificity
- Leu31-Thr172
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Cripto at 2 μg/mL (100 μL/well) can bind Recombinant Human ALK4 with a linear range of 0.15-151 ng/mL.
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Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) (AA 31-150) protein (His tag)
TDGF1.3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- Cripto-1 (TDGF1.3) (Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3))
- Alternative Name
- Cripto-1/TDGF1 (TDGF1.3 Products)
- Background
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Description: Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. TDGF1 is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. It is overexpressed in many types of cancers and acts as a growth factor for tumors. Cripto mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants. Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1.
Name: TDGF1,CR,CRGF,CRIPTO
- Gene ID
- 6997
- UniProt
- P13385
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