TNFRSF12A Protein (Fc Tag,His tag)

Catalog No. ABIN7520592

Product Details

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF12A protein is labelled with Fc Tag,His tag.

Purpose: Active Recombinant Human TNFRSF12A/TWEAKR/CD266 Protein

Sequence: EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LW

Specificity: Glu28-Trp79

Purity: > 90 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its ability to inhibit TWEAK-induced apoptosis in HT-29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 2-12 μg/mL in the presence of 1 μg/mL recombinant human TWEAK.|2.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL.|3.Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Alternative Name: TNFRSF12A/TWEAKR/CD266 (TNFRSF12A Products)

Synonyms: CD266 Protein, FN14 Protein, TWEAKR Protein, AI255180 Protein, C87282 Protein, Fn14 Protein, HPIP Protein, TWEAK-R Protein, TweakR Protein, TNF receptor superfamily member 12A Protein, tumor necrosis factor receptor superfamily, member 12a Protein, TNFRSF12A Protein, Tnfrsf12a Protein

Background:

Description: Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Human and mouse TNFRSF12A share 82 % aa sequence identity. TNFRSF12A transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated TNFRSF12A expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. TNFRSF12A is the weak inducer of apoptosis in some cell types. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.

Name: CD266, FN14, TWEAKR,TNFRSF12A,FN14,TWEAKR

Gene ID: 51330

UniProt: Q9NP84

Pathways: Apoptosis, Regulation of Cell Size