IL-1 beta Protein (AA 118-269)

Catalog No. ABIN7669002

Recombinant IL-1 beta protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-1 beta Protein (AA 118-269) (ABIN7669002)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Protein Characteristics: AA 118-269

Purpose: Recombinant Mouse IL-1 beta/IL1B Protein

Sequence: Val118-Ser269

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1 beta,IL1B

Background: Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25 % homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1β, designated interleukin 1β-converting enzyme (ICE), has been described. Mature human and mouse IL-1β share approximately 75 % amino acid sequence identity and human IL-1β has been found to be active on murine cell lines.,IL1F,catabolin,Il-1b,IL1-BETA,IL-1beta,IL1F2,Il1b,Interleukin-1 beta,IL-1 beta

Molecular Weight:

calculated_mw: 17.4 kDa

observed_mw: 16 kDa

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome