SIRT1 Protein (GST tag)

Catalog No. ABIN7857312

This Recombinant SIRT1 protein is produced in Escherichia coli (E. coli).

Quick Overview for SIRT1 Protein (GST tag) (ABIN7857312)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Product Details

Purification tag / Conjugate: This SIRT1 protein is labelled with GST tag.

Purpose: Recombinant SIRT1 Protein

Sequence: 193-end

Characteristics: Recombinant human SIRT1 (193-end) was expressed in E. coli cells using an N-terminal GST tag.

Sterility: Sterile

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, 10-25 % glycerol

Preservative: Dithiothreitol (DTT), PMSF

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Expiry Date: 12 months

Target Details

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Molecular Weight: ~105 kDa

NCBI Accession: NM_012238

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling