TNF alpha Protein
Quick Overview for TNF alpha Protein (ABIN7937245)
Target
See all TNF alpha ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Rhesus Macaque Tumor Necrosis Factor-alpha/TNFSF2
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Sequence
- VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 1 EU/ug of rRhTNF-alpha as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg in the presence of actinomycin D.
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Comment
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Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- Tumor Necrosis Factor-alpha
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Molecular Weight
- 17.3 kDa
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Gene ID
- 715467
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UniProt
- P48094
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Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Target
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