p21 Protein

Catalog No. ABIN7938737

This Recombinant p21 protein is produced in Escherichia coli (E. coli).

Quick Overview for p21 Protein (ABIN7938737)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant CDKN1A protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Recombinant CDKN1A protein is in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A

Background:

Protein Background: CDKN1A (Cyclin-dependent kinase inhibitor 1) may be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest.

Short Description: Recombinant CDKN1A protein that includes amino acids 1-164 of human CDKN1A protein (accession number NP_000380.1) was expressed was expressed in E. coli cells with an N-terminal 6x His tag. The molecular weight of the protein is 20.05 kDa.

Aliases: Cyclin-dependent kinase inhibitor 1, also known as P21, CIP1, CDK1A, P21CIP1

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy