E2F1 Protein
Quick Overview for E2F1 Protein (ABIN7938780)
Target
See all E2F1 ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant E2F1 protein
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E2F Transcription Factor 1 (E2F1) protein (Myc-DYKDDDDK Tag)
E2F1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant E2F1 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- E2F1 (E2F Transcription Factor 1 (E2F1))
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Alternative Name
- E2F1
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Background
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Protein Background: E2F1 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/ p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters.
Short Description: Recombinant E2F1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_005216.1) with an N-terminal FLAG tag. The molecular weight of E2F1 is 48.19 kDa.
Aliases: E2F Transcription Factor 1, RBBP3, RBP3, Transcription Factor E2F1, RBAP-1, E2F-1, PBR3
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Pathways
- p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
Target
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