E2F1 Protein (Myc-DYKDDDDK Tag)
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- Target See all E2F1 Proteins
- E2F1 (E2F Transcription Factor 1 (E2F1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This E2F1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human E2F1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product E2F1 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression." in: Nature communications, Vol. 6, pp. 10028, (2015) (PubMed).
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Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression." in: Nature communications, Vol. 6, pp. 10028, (2015) (PubMed).
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- Target
- E2F1 (E2F Transcription Factor 1 (E2F1))
- Alternative Name
- e2f1 (E2F1 Products)
- Synonyms
- CG6376 Protein, DRTF1/E2F Protein, DmE2F-1 Protein, Dmel\\CG6376 Protein, Dp Protein, E(Sev-CycE)3A Protein, E(var)3-93E Protein, E(var)3-95E Protein, E(var)93E Protein, E2F Protein, E2F-1 Protein, E2F1 Protein, E2f1 Protein, Evar(3)164 Protein, dE2F Protein, dE2F1 Protein, dE2f Protein, dE2f1 Protein, de2f1 Protein, def21 Protein, drosE2F1 Protein, e2f Protein, e2f1 Protein, l(3)07172 Protein, l(3)j3B1 Protein, l(3)j3C2 Protein, l(3)rM729 Protein, RBAP1 Protein, RBBP3 Protein, RBP3 Protein, mKIAA4009 Protein, xE2F Protein, ATE2FB Protein, E2F TRANSCRIPTION FACTOR-1 E2F1 Protein, E2F transcription factor 1 Protein, E2FB Protein, E2FB TRANSCRIPTION FACTOR Protein, E2F transcription factor 1 Protein, E2F transcription factor 1 L homeolog Protein, E2f1 Protein, E2F1 Protein, e2f1.L Protein
- Background
- The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
- Molecular Weight
- 46.7 kDa
- NCBI Accession
- NP_005216
- Pathways
- p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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