KIT Protein (AA 546-976)

Catalog No. ABIN7939264

This Recombinant KIT protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for KIT Protein (AA 546-976) (ABIN7939264)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Product Details

Protein Characteristics: AA 546-976

Purpose: Recombinant KIT / CD117 (546-976) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant KIT (546-976) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: KIT & CD117

Background:

Protein Background: KIT (KIT Proto-Oncogene, Receptor Tyrosine Kinase) is a tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. KIT phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL and activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KIT promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Diseases associated with KIT include Gastrointestinal Stromal Tumor and Piebald Trait.

Short Description: Recombinant KIT (546-976) protein that includes amino acids 546-976 of human KIT protein (accession number NP_000213.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 50 kDa.

Aliases: KIT Proto-Oncogene, Receptor Tyrosine Kinase, CD117

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity