MAP2K6 Protein

Catalog No. ABIN7939335

This Recombinant MAP2K6 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for MAP2K6 Protein (ABIN7939335)

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant MAP2K6 (MEK6) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MAP2K6 (MEK6) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Alternative Name: MAP2K6

Background:

Protein Background: MAP2K6 (Dual specificity mitogen-activated protein kinase kinase 6), also known as MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3 and SAPKK3.MAP2K6 is a dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction, and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.

Short Description: Recombinant MAP2K6 (MEK6) protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_002749.2) with an N-terminal FLAG tag. The molecular weight of the protein is 38.78 kDa.

Aliases: Mitogen activated protein kinase kinase 6, SAPKK3, SAPKK-3, MEK6, MKK6, MAPKK6, PRKMK6, MAPK/ERK Kinase 6, SAPK Kinase, MEK

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades