IL-2 Protein (AA 21-155)

Catalog No. ABIN8057018

Recombinant IL-2 protein expressed in HEK-293 Cells.

Quick Overview for IL-2 Protein (AA 21-155) (ABIN8057018)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Application: Immunogen (Imm)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-155

Purpose: Recombinant Rat IL-2 Protein(Halo Tag)

Sequence: Ala21-Gln155

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: T-cell growth factor,TCGF,Aldesleukin,Interleukin-2(IL-2) is a O-glycosylated four α-helix bundle cytokine that has potent stimulatory activity for antigenactivated T cells. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. Mature rat IL-2 shares 66 % and 73 % amino acid sequence identity with human and mouse IL-2,respectively. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.

Molecular Weight:

Calculated MW: 56.7 kDa

Observed MW: 45-50 kDa

Gene ID: 116562

UniProt: P17108

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation