BDNF Protein (AA 139-249)
Quick Overview for BDNF Protein (AA 139-249) (ABIN8127014)
Target
See all BDNF ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 139-249
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Purpose
- Active Brain Derived Neurotrophic Factor (BDNF)
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Sequence
- Glu139-Arg249
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Brain-Derived Neurotrophic Factor (BDNF) (Active) protein (Biotin)
BDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant WB, CC, in vivo, Diff, AD, CyTox, LCI, IF, FM Active
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-255) protein (GST tag)BDNF Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) protein (GST tag)BDNF Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 8.5
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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Alternative Name
- Brain Derived Neurotrophic Factor
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Background
- Neurotrophin, Abrineurin
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Molecular Weight
- 45kDa
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UniProt
- P21237
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Pathways
- RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
Target
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