TNF alpha Protein (AA 71-234)
Quick Overview for TNF alpha Protein (AA 71-234) (ABIN8129563)
Target
See all TNF alpha ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 71-234
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Purpose
- Active Tumor Necrosis Factor Alpha (TNFa)
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Sequence
- Asn71-Leu234
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin,His-Avi Tag)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 78-233) protein (His tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His-Avi Tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 78-235) (Active) proteinTNF alpha Origin: Rabbit Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. CC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (GST tag)TNF alpha Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Tumor Necrosis Factor alpha (TNF alpha) (AA 89-235) (Active) proteinTNF alpha Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. CC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) (Active) protein (His tag)TNF alpha Origin: Rat Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 5.6
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 20 mM Tris, 150 mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- Tumor Necrosis Factor Alpha
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Background
- DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
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Molecular Weight
- 22kDa
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UniProt
- Q06599
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Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Target
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