Leptin Protein (LEP)
Quick Overview for Leptin Protein (LEP) (ABIN935617)
Target
See all Leptin (LEP) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC
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Characteristics
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Purified recombinant Human Leptin protein
Expression System: E.coli
Bioactivity: Determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leptin (LEP) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) protein (His tag)LEP Origin: Goldfish Host: Yeast Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 428-635) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leptin (LEP) proteinLEP Origin: Rat Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 1.0-5.0 mg/mL.
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Buffer
- Lyophilized from 10 mM sodium citrate, pH 4.0.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- Leptin (LEP)
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Alternative Name
- Leptin
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Background
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Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models.
Alternative Names: Obesity protein, OB protein -
Molecular Weight
- 16.2 kDa
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Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target
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