Thymic Stromal Lymphopoietin Protein (TSLP)

Catalog No. ABIN988257

This Thymic Stromal Lymphopoietin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Thymic Stromal Lymphopoietin Protein (TSLP) (ABIN988257)

Target: Thymic Stromal Lymphopoietin (TSLP)

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Product Details

Sequence: MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC L EQVSQLQG L WRRFNRPL LK Q

Characteristics: Fully biologically active when compared to standard. The ED50 as measured by its ability to induce proliferation of human IL-7Rª and human TSLP R co-transfected mouse BaF3 cells is less than 0.3 ng/ml,corresponding to a specific activity of > 3.3×106 units/mg.

Endotoxin Level: Level Less than 1EU/µg of rHuTSLP as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Background: TSLP is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rª) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naive T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFª) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation. Synonym: Thymic Stromal Lymphopoietin ( TSLP), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Molecular Weight: Approximately 15.0 KDa, a single non-glycosylated polypeptide chain containing 132 amino acids.