anti-Human ARNT antibody for Western Blotting

Recommended ARNT Antibody (supplied by: Log in to see )

Antigen
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies
  • HIF-1-beta
  • HIF-1beta
  • HIF1-beta
  • HIF1B
  • HIF1BETA
  • TANGO
  • bHLHe2
  • D3Ertd557e
  • Drnt
  • ESTM42
  • Hif1b
  • W08714
  • mKIAA4051
  • Arnt1
  • ARNT
  • zgc:136664
  • arnt1
  • hif1b
  • tango
  • xArnt
  • hif1beta
  • hif-1beta
  • arnt.2
  • MGC68805
  • aryl hydrocarbon receptor nuclear translocator
  • aryl hydrocarbon receptor nuclear translocator S homeolog
  • ARNT
  • Arnt
  • arnt
  • arnt.S
Reactivity
Cow (Bovine), Ferret, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine)
163
73
66
31
25
20
18
8
8
7
7
6
6
3
2
1
1
Host
Mouse
82
60
28
Clonality
Monoclonal
Conjugate
This ARNT antibody is un-conjugated
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
144
67
60
47
38
19
19
10
7
6
6
4
3
3
2
1
1
Supplier
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Catalog No. ABIN151055
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2014155 ABIN151037 ChIP GS ICC IF IHC IHC (p) IP SimWes WB Rabbit Log in to see Polyclonal 34
3.2014155 ABIN152684 GS ICC IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 2B10 10
3.2014155 ABIN2777195 WB Rabbit C-Term Log in to see Polyclonal 1
3.2014155 ABIN2777194 WB Rabbit N-Term Log in to see Polyclonal 0
3.2014155 ABIN2774828 WB Rabbit Middle Region Log in to see Polyclonal 0
3.2014155 ABIN968356 IF WB Mouse IgG1 AA 461-574 Log in to see OPG-01 6
3.2014155 ABIN2707549 IHC WB Rabbit N-Term Log in to see Polyclonal 0
3.2014155 ABIN3043528 WB Rabbit IgG AA 416-789 Log in to see Polyclonal 0
3.2014155 ABIN1533680 ELISA WB Rabbit IgG AA 31-80 Log in to see Polyclonal 1
3.2014155 ABIN658231 IF WB Rabbit Ig Fraction AA 513-544, Center, Val528 Log in to see Polyclonal 2
3.2014155 ABIN394250 IF WB Mouse IgG2a kappa AA 1-110 Log in to see 3D10 5
3.2014155 ABIN782335 EIA IF IHC (p) WB Mouse IgG2a AA 1-110 Log in to see 3D10 0
3.2014155 ABIN968355 IF WB Mouse IgG1 AA 461-574 Log in to see 29-HIF 5
3.2014155 ABIN513506 IF WB Mouse AA 1-789, full length Log in to see Polyclonal 1
3.2014155 ABIN1871099 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2014155 ABIN559953 IF IHC (p) RNAi ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 3D10 0
3.2014155 ABIN1866774 ICC IHC WB Rabbit IgG AA 624-789 Log in to see Polyclonal 0
3.2014155 ABIN3185004 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.2014155 ABIN5609834 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.2014155 ABIN5609835 ELISA WB Goat Internal Region Log in to see Polyclonal 0

General

Antigen Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies
Reactivity Cow (Bovine), Ferret, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine)
(163), (73), (66), (31), (25), (20), (18), (8), (8), (7), (7), (6), (6), (3), (2), (1), (1)
Host Mouse
(82), (60), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ARNT antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(144), (67), (60), (47), (38), (19), (19), (10), (7), (6), (6), (4), (3), (3), (2), (1), (1)
Pubmed 89 references available
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Product Details anti-ARNT Antibody

Target Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN151055) Images
Purification Protein G purified
Immunogen Fusion protein containing amino acids 496-789 of human HIF-1 beta. [UniProt# P27540]
Clone H1beta234
Isotype IgG1 kappa

Target Details ARNT

Product Details anti-ARNT Antibody Application Details Handling References for anti-ARNT antibody (ABIN151055) Images back to top
Antigen
Alternative Name ARNT/HIF-1 beta (ARNT Antibody Abstract)
Background Gene Symbol: ARNT
Molecular Weight Theoretical MW: 92 kDa
Gene ID 405
UniProt P27540
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-ARNT Antibody Target Details ARNT Handling References for anti-ARNT antibody (ABIN151055) Images back to top
Application Notes Western Blot 1:500-1:2000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:100, Gel Super Shift AssaysThis HIF-1 beta (H1beta234) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and Chromatin Immunoprecipitation. By Western blot a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839).Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25343232) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HIF-1 beta Antibody Western Blot Procedure
1. Resolve aliquots (15 mg) of induced nuclear protein extracts on a SDS/6 % polyacrylamide gel.
. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol.
. Block membranes for 1.5 hours with 1X western wash buffer containing 5 % non-fat dry milk (NFDM).
. Incubate membranes for 1.5 hours at room temperature (RT) in NB 100-124 diluted 1:1,500 in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Incubate membranes with HRP conjugated anti-Mouse IgG for 1 hour (RT) in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Drain membrane and place on saran wrap.
. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds.
. Drain membrane and place on new saran wrap
. Wrap up membrane and expose to film.
. Develop accordingly.10X Western wash 24.2g Tris80g NaCl Tween-20 to 1 % pH 7.6 and QS to 4LStripping buffer100 mM BME2 % SDS62.5 mM Tris (pH 6.7)Incubate membrane for 30 minutes at 56 degrees C. Wash membrane 15 minutes with several changes of 1X western wash.
Restrictions For Research Use only

Handling

Product Details anti-ARNT Antibody Target Details ARNT Application Details References for anti-ARNT antibody (ABIN151055) Images back to top
Format Liquid
Concentration 1.4 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-ARNT antibody (ABIN151055)

Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling Images back to top
Product cited in:

Murakami, Wang, Kalhorn, Schraml, Rathmell, Tan, Nemenoff, Stenmark, Jiang, Reyland, Heasley, Hu: "Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma." in: Oncogenesis, Vol. 6, Issue 1, pp. e287, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Lu, Chen, Shimoda, Park, Zhang, Tran, Zhang, Semenza: "Chemotherapy-Induced Ca(2+) Release Stimulates Breast Cancer Stem Cell Enrichment." in: Cell reports, Vol. 18, Issue 8, pp. 1946-1957, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Li, Heller, Bostick, Lee, Schjerven, Kastner, Chan, Chen, Zhou: "Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway." in: Immunity, Vol. 45, Issue 1, pp. 185-97, 2016 (Sample species: Human). Further details: Western Blotting

Thienpont, Steinbacher, Zhao, DAnna, Kuchnio, Ploumakis, Ghesquière, Van Dyck, Boeckx, Schoonjans, Hermans, Amant, Kristensen, Koh, Mazzone, Coleman, Carell, Carmeliet, Lambrechts: "Tumour hypoxia causes DNA hypermethylation by reducing TET activity." in: Nature, Vol. 537, Issue 7618, pp. 63-68, 2016 (Sample species: Mouse (Murine)). Further details: Chromatin Immunoprecipitation

Taylor, Bagnall, Mason, Levy, Fernig, See: "Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility." in: Open biology, Vol. 6, Issue 9, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Altuntas, Rossin, Marsella, DEletto, Diaz-Hidalgo, Farrace, Campanella, Antonioli, Fimia, Piacentini: "The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation." in: Oncotarget, Vol. 6, Issue 42, pp. 44941-54, 2016 Method employed by authors: Western Blotting (WB)

Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 Method employed by authors: Western Blotting (WB)

Rossin, DEletto, Falasca, Sepe, Cocco, Fimia, Campanella, Mastroberardino, Farrace, Piacentini: "Transglutaminase 2 ablation leads to mitophagy impairment associated with a metabolic shift towards aerobic glycolysis." in: Cell death and differentiation, Vol. 22, Issue 3, pp. 408-18, 2015 Further details: Western Blotting

Kim, Kim, Park, Jung, Cho, Kim, Han, Choi, Kwon: "NNC 55-0396, a T-type Ca2+ channel inhibitor, inhibits angiogenesis via suppression of hypoxia-inducible factor-1α signal transduction." in: Journal of molecular medicine (Berlin, Germany), Vol. 93, Issue 5, pp. 499-509, 2015 (Sample species: Human). Further details: Western Blotting

Xiang, Gilkes, Hu, Luo, Bullen, Liang, Semenza: "HIF-1α and TAZ serve as reciprocal co-activators in human breast cancer cells." in: Oncotarget, Vol. 6, Issue 14, pp. 11768-78, 2015 (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation,Immunoprecipitation

Su, Xin, Yin, Dyson, Coon, Farrow, Mestan, Ernst: "Impaired fetoplacental angiogenesis in growth-restricted fetuses with abnormal umbilical artery doppler velocimetry is mediated by aryl hydrocarbon receptor nuclear translocator (ARNT)." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 1, pp. E30-40, 2015 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Xiang, Gilkes, Hu, Takano, Luo, Lu, Bullen, Samanta, Liang, Semenza: "Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype." in: Oncotarget, Vol. 5, Issue 24, pp. 12509-27, 2015 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Frey, Stonko, Faugere, Riddle: "Hypoxia-inducible factor-1α restricts the anabolic actions of parathyroid hormone." in: Bone research, Vol. 2, pp. 14005, 2015 Method employed by authors: Immunofluorescence (IF)

Sena, Wang, Pawlus, Hu: "HIFs enhance the transcriptional activation and splicing of adrenomedullin." in: Molecular cancer research : MCR, Vol. 12, Issue 5, pp. 728-41, 2014 (Sample species: Human). Further details: Western Blotting

Wang, Gilkes, Takano, Xiang, Luo, Bishop, Chaturvedi, Green, Semenza: "Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 31, pp. E3234-42, 2014 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Peng, Yuan, Khan, Nanduri, Makarenko, Reddy, Vasavda, Kumar, Semenza, Prabhakar: "Regulation of hypoxia-inducible factor-α isoforms and redox state by carotid body neural activity in rats." in: The Journal of physiology, Vol. 592, Issue 17, pp. 3841-58, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting

Pawlus, Wang, Hu: "STAT3 and HIF1α cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells." in: Oncogene, Vol. 33, Issue 13, pp. 1670-9, 2014 (Sample species: Human). Further details: Western Blotting

Xiang, Gilkes, Chaturvedi, Luo, Hu, Takano, Liang, Semenza: "Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 2, pp. 151-64, 2014 (Sample species: Human). Further details: Western Blotting

Gilkes, Xiang, Lee, Chaturvedi, Hubbi, Wirtz, Semenza: "Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 3, pp. E384-93, 2014 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Takano, Peng, Kumar, Luo, Hu, Shimoda, Suematsu, Prabhakar, Semenza: "Hypoxia-inducible factors regulate human and rat cystathionine ?-synthase gene expression." in: The Biochemical journal, Vol. 458, Issue 2, pp. 203-11, 2014 Further details: Chromatin Immunoprecipitation

Images

Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN151055) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN151055) Immunohistochemical staining of human glioblastoma multi-forme utilizing anti-HIF-1 B...
Western Blotting (WB) image for anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (ABIN151055) Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [ABIN151055] - Analysis of HIF-1 b...