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|Antigen||Bone Morphogenetic Protein 2 (BMP2) Antibodies|
|Epitope||Internal Region, AA 250-350 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BMP2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|8 references available|
|Supplier||Log in to see|
Product Details anti-BMP2 AntibodyTarget Details BMP2 Application Details Handling References for anti-BMP2 antibody (ABIN446957) Images
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an internal region of human BMP2 (within residues 250-350) [Swiss-Prot# P12643]|
|Plasmids, Primers & others|
Target Details BMP2Product Details anti-BMP2 Antibody Application Details Handling References for anti-BMP2 antibody (ABIN446957) Images back to top
|Alternative Name||BMP-2 (BMP2 Antibody Abstract)|
|Background||Gene Symbol: BMP2|
|Molecular Weight||Theoretical MW: 44 kDa|
|Pathways||Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-BMP2 Antibody Target Details BMP2 Handling References for anti-BMP2 antibody (ABIN446957) Images back to top
|Application Notes||Western Blot 0.5 μg/mL, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200This BMP2 antibody is useful for Immunohistochemistry-Paraffin and Western Blot, where a band is seen ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western Blot protocol specific for BMP2 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Protocol Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BMP2 Antibody Target Details BMP2 Application Details References for anti-BMP2 antibody (ABIN446957) Images back to top
PBS ( pH 7.4)
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-BMP2 antibody (ABIN446957)Product Details anti-BMP2 Antibody Target Details BMP2 Application Details Handling Images back to top
|Product cited in:||
Scimeca, Piccirilli, Mastrangeli, Rao, Feola, Orlandi, Gasbarra, Bonanno, Tarantino: "Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 34, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).
Cordner, Friend, Mayo, Badgley, Wallmann, Stallings, Young, Miles, Edwards, Bridgewater: "The BMP2 nuclear variant, nBMP2, is expressed in mouse hippocampus and impacts memory." in: Scientific reports, Vol. 7, pp. 46464, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).
Gullard, Croney, Wu, Mamaeva, Sohn, Cao, MacDougall: "Reduced Dentin Matrix Protein Expression in Camurati-Engelmann Disease Transgenic Mouse Model." in: Journal of cellular physiology, Vol. 231, Issue 5, pp. 1106-13, 2016 (Sample species: Mouse (Murine)).
Chen, Choesang, Li, Sun, Pham, Bao, Feola, Westerman, Li, Follenzi, Blanc, Rivella, Fleming, Ginzburg: "Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation." in: Haematologica, Vol. 101, Issue 3, pp. 297-308, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting
Scimeca, Bonanno, Piccirilli, Baldi, Mauriello, Orlandi, Tancredi, Gasbarra, Tarantino: "Satellite Cells CD44 Positive Drive Muscle Regeneration in Osteoarthritis Patients." in: Stem cells international, Vol. 2015, pp. 469459, 2015 Further details: Immunohistochemistry
Yeom, Kim, Lee, Zhang, Lee, Hahm, Sohn, Lee: "Effects of laser acupuncture on longitudinal bone growth in adolescent rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 424587, 2013 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Bridgewater, Mayo, Evanson, Whitt, Dean, Yates, Holden, Schmidt, Fox, Dhunghel, Steed, Adam, Nichols, Loganathan, Barrow, Hancock: "A novel bone morphogenetic protein 2 mutant mouse, nBmp2NLS(tm), displays impaired intracellular Ca2+ handling in skeletal muscle." in: BioMed research international, Vol. 2013, pp. 125492, 2013 (Sample species: Mouse (Murine)).
Kahn, Bosch, Levitt, Goldstein: "Effect of sodium nitrate loading on electrolyte transport by the renal tubule." in: The American journal of physiology, Vol. 229, Issue 3, pp. 746-53, 1976 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).
ImagesProduct Details anti-BMP2 Antibody Target Details BMP2 Application Details Handling References for anti-BMP2 antibody (ABIN446957) back to top