anti-Human PR Domain Containing 1, with ZNF Domain antibody for Western Blotting

Recommended PR Domain Containing 1, with ZNF Domain Antibody (supplied by: Log in to see )

Antigen
PR Domain Containing 1, with ZNF Domain (PRDM1) Antibodies
  • GB11750
  • CG5249
  • Dmel\\CG5249
  • blimp-1
  • dBlimp-1
  • dBlimp1
  • Blimp-1
  • Blimp1
  • nrd
  • prdm1
  • ubo
  • wu:fb99c12
  • zgc:91885
  • MGC52536
  • PRDM1
  • blimp1
  • xblimp1
  • prdi-bf1
  • BLIMP1
  • PRDI-BF1
  • ZNFPR1A1
  • PR domain containing 1, with ZNF domain
  • Blimp-1 protein
  • CG5249 gene product from transcript CG5249-RB
  • blimp-1
  • PR domain containing 1a, with ZNF domain
  • B lymphocyte induced maturation protein 1
  • PRDM1
  • LOC100338324
  • Blimp-1
  • CpipJ_CPIJ007425
  • prdm1a
  • prdm1
  • Prdm1
Epitope
Middle Region
29
21
16
12
11
9
9
8
7
7
7
5
5
5
5
4
3
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
177
72
37
27
12
9
4
3
3
3
2
2
1
Host
Rabbit
81
72
33
3
Clonality
Polyclonal
Conjugate
Un-conjugated
10
9
8
6
5
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
168
125
33
32
31
30
24
16
10
7
3
2
2
1
1
1
1
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Catalog No. ABIN2792524
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1469584 ABIN2780353 WB Rabbit N-Term Log in to see Polyclonal 1
3.1469584 ABIN153174 ChIP ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 AA 199-409 Log in to see 3H2-E8 43
3.1469584 ABIN964913 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
3.1469584 ABIN964914 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
3.1469584 ABIN4284761 WB Rabbit Log in to see Polyclonal 1
3.1469584 ABIN4284751 ELISA IHC IHC (p) WB Goat both Isoforms Log in to see Polyclonal 1
3.1469584 ABIN4284750 ELISA IHC WB Goat C-Term, both Isoforms Log in to see Polyclonal 1
3.1469584 ABIN1724737 ELISA WB Mouse IgG1 Log in to see 5-00E-07 3
3.1469584 ABIN2464857 ELISA WB Goat Internal Region Log in to see Polyclonal
3.1469584 ABIN2464801 ELISA WB Goat C-Term Log in to see Polyclonal
3.1469584 ABIN658442 WB Rabbit Ig AA 106-134, N-Term Log in to see Polyclonal
3.1469584 ABIN394172 WB Mouse IgG1 kappa AA 1-110 Log in to see 2F8
3.1469584 ABIN394106 WB Mouse IgG2a kappa AA 1-110 Log in to see 2B10
3.1469584 ABIN2461625 ELISA WB Rabbit Log in to see Polyclonal
3.1469584 ABIN1030298 IF ELISA WB Rabbit C-Term Log in to see Polyclonal
3.1469584 ABIN1031276 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.1469584 ABIN2668251 WB Rabbit IgG N-Term Log in to see Polyclonal
3.1469584 ABIN1171717 ICC IHC IP WB Rabbit IgG AA 83-203 Log in to see Polyclonal
3.1469584 ABIN560062 RNAi ELISA WB Mouse IgG2a kappa AA 1-109, partial Log in to see 2B10
3.1469584 ABIN513732 IF ELISA WB Mouse IgG2a kappa AA 422-493, full length Log in to see 1G9

General

Antigen PR Domain Containing 1, with ZNF Domain (PRDM1) Antibodies
Epitope Middle Region
(29), (21), (16), (12), (11), (9), (9), (8), (7), (7), (7), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(177), (72), (37), (27), (12), (9), (4), (3), (3), (3), (2), (2), (1)
Host Rabbit
(81), (72), (33), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(10), (9), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(168), (125), (33), (32), (31), (30), (24), (16), (10), (7), (3), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product details

Target details Application Details Handling References Images
Sequence VEDDISVISV VEKEILAVVR KEKEETGLKV SLQRNMGNGL LSSGCSLYES
Cross-Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 84%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 92%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against PRDM1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PRDM1

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name PRDM1 / BLIMP1 (PRDM1 Antibody Abstract)
Background PRDM1 is a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Alias Symbols: BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1
Protein Interaction Partner: PRMT5, SENP1, EHMT2, PIAS1, SUMO1, IRF2, IRF1, HDAC2, HDAC1, HIST1H1A, MS4A1, HSP90AA1, ATXN1, ELAVL1, KDM1A, IRF4, TLE2, TLE1, AES, PRMT7, HIST2H3A,
Protein Size: 691
Molecular Weight 77 kDa
Gene ID 639
NCBI Accession NM_182907, NP_878911
UniProt Q86WM7
Pathways Regulation of Muscle Cell Differentiation

Application Details

Product details Target details Handling References Images back to top
Application Notes WB Suggested Anti-PRDM1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: SH-SYSY cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 691 AA

Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Product cited in:

Arulanandam, Batenchuk, Angarita, Ottolino-Perry, Cousineau, Mottashed, Burgess, Falls, De Silva, Tsang, Howe, Bourgeois-Daigneault, Conrad, Daneshmand, Breitbach, Kirn, Raptis, Sad, Atkins, Huh et al.: "VEGF-Mediated Induction of PRD1-BF1/Blimp1 Expression Sensitizes Tumor Vasculature to Oncolytic Virus Infection. ..." in: Cancer cell, Vol. 28, Issue 2, pp. 210-24, 2015

Background publications

Diehl, Schmidlin, Nagasawa, van Haren, Kwakkenbos, Yasuda, Beaumont, Scheeren, Spits: "STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4805-15, 2008

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (Middle Region) antibody (ABIN2792524) WB Suggested Anti-PRDM1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (Middle Region) antibody (ABIN2792524) anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (Middle Region) antibody (ABIN2792524) anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (Middle Region) antibody (Image 3)