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|Antigen||Argininosuccinate Synthase 1 (ASS1) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Cow (Bovine), Human Alternatives|
|Conjugate||This ASS1 antibody is un-conjugated Alternatives|
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
Product Details anti-ASS1 AntibodyTarget Details ASS1 Application Details Handling References for anti-ASS1 antibody (ABIN190834) Images
|Purpose||Argininosuccinate synthetase 1|
|Blocking Peptide||Blocking peptide for this product available: ABIN369030|
|Specificity||The variants represent identical protein (NP_000041.2 and NP_446464.1).|
|Predicted Reactivity||Human, Mouse, Rat, Dog|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Plasmids, Primers & others|
Target Details ASS1Product Details anti-ASS1 Antibody Application Details Handling References for anti-ASS1 antibody (ABIN190834) Images back to top
|Alternative Name||ASS1 (ASS1 Antibody Abstract)|
|Background||Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase|
|Pathways||Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-ASS1 Antibody Target Details ASS1 Handling References for anti-ASS1 antibody (ABIN190834) Images back to top
DS WB Results: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of 46.5 kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.
DS IHC Results: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.
Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.
Immunofluorescence: A customer has reported this antibody works well in IF.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ASS1 Antibody Target Details ASS1 Application Details References for anti-ASS1 antibody (ABIN190834) Images back to top
|Buffer||Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Minimize freezing and thawing.|
|Storage Comment||Aliquot and store at -20 °C.|
References for anti-ASS1 antibody (ABIN190834)Product Details anti-ASS1 Antibody Target Details ASS1 Application Details Handling Images back to top
|Product cited in:||
Corbin, Pendleton, Solomonson, Eichler: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, 2008
Hao, Xie, Gross: "Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36192-200, 2004
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