anti-Cow (Bovine) ASS1 antibody for Immunohistochemistry

Recommended ASS1 Antibody (supplied by: Log in to see )

Antigen
Argininosuccinate Synthase 1 (ASS1) Antibodies
  • ASS
  • CTLN1
  • AA408052
  • Ass-1
  • fold
  • ASSA
  • Ass
  • ass
  • zgc:92051
  • wu:fb95a04
  • wu:fc01e08
  • argininosuccinate synthase 1
  • argininosuccinate synthetase 1
  • argininosuccinate synthase 1 S homeolog
  • ASS1
  • Ass1
  • ass1.S
  • ass1
Epitope
Internal Region
28
11
11
8
5
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Human
121
35
24
13
11
10
4
3
2
2
2
1
1
1
Host
Goat
63
33
25
Clonality
Polyclonal
Conjugate
This ASS1 antibody is un-conjugated
6
4
4
3
3
3
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
111
71
59
32
30
25
22
10
6
5
1
1
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Catalog No. ABIN190834
$ 429.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.492902 ABIN2776769 IHC WB Rabbit C-Term Log in to see Polyclonal 2
12.992902 ABIN489997 ELISA IHC IHC (p) IP WB Goat AA 196-212 Log in to see Polyclonal 0
12.758589 ABIN5539685 ELISA IF IHC IP WB Biotin Goat Internal Region Log in to see Polyclonal 0
9.992902 ABIN2776768 IHC WB Rabbit N-Term Log in to see Polyclonal 1
9.992902 ABIN5894198 ELISA IHC WB Goat Log in to see Polyclonal 0
7 ABIN320629 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
4 ABIN2466477 IF IP IHC ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN4950203 ELISA IF IHC IP WB Goat Ig Fraction Log in to see Polyclonal 0

General

Antigen Argininosuccinate Synthase 1 (ASS1) Antibodies
Epitope Internal Region
(28), (11), (11), (8), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human
(121), (35), (24), (13), (11), (10), (4), (3), (2), (2), (2), (1), (1), (1)
Host Goat
(63), (33), (25)
Clonality
Polyclonal
Conjugate This ASS1 antibody is un-conjugated
(6), (4), (4), (3), (3), (3)
Application ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(111), (71), (59), (32), (30), (25), (22), (10), (6), (5), (1), (1)
Pubmed 2 references available
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Product Details anti-ASS1 Antibody

Target Details ASS1 Application Details Handling References for anti-ASS1 antibody (ABIN190834) Images
Purpose Argininosuccinate synthetase 1
Blocking Peptide Blocking peptide for this product available: ABIN369030
Sequence ENPKNQAPPG LYTKTQD
Specificity The variants represent identical protein (NP_000041.2 and NP_446464.1).
Predicted Reactivity Human, Mouse, Rat, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen C-ENPKNQAPPGLYTKTQD
Plasmids, Primers & others

Target Details ASS1

Product Details anti-ASS1 Antibody Application Details Handling References for anti-ASS1 antibody (ABIN190834) Images back to top
Antigen
Alternative Name ASS1 (ASS1 Antibody Abstract)
Background Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
Gene ID 445
NCBI Accession NP_000041
Pathways Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-ASS1 Antibody Target Details ASS1 Handling References for anti-ASS1 antibody (ABIN190834) Images back to top
Application Notes

DS WB Results: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of 46.5 kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Comment

Immunofluorescence: A customer has reported this antibody works well in IF.

Restrictions For Research Use only

Handling

Product Details anti-ASS1 Antibody Target Details ASS1 Application Details References for anti-ASS1 antibody (ABIN190834) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-ASS1 antibody (ABIN190834)

Product Details anti-ASS1 Antibody Target Details ASS1 Application Details Handling Images back to top
Product cited in:

Corbin, Pendleton, Solomonson, Eichler: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, 2008

Background publications

Hao, Xie, Gross: "Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36192-200, 2004

Images

Product Details anti-ASS1 Antibody Target Details ASS1 Application Details Handling References for anti-ASS1 antibody (ABIN190834) back to top
Supplier Images
 image for anti-Argininosuccinate Synthase 1 (ASS1) (Internal Region) antibody (ABIN190834) ABIN190834 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer)....