anti-Human Nucleolin antibody for ELISA

Recommended Nucleolin Antibody (supplied by: Log in to see )

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human
232
56
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
125
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
16
11
10
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
217
134
124
91
63
57
41
24
21
17
13
9
2
1
1
Options
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Available images

Catalog No. ABIN3032071
$ 452.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16 ABIN3032072 IF IHC ELISA WB Mouse IgG1 Log in to see 130CT43-8-5 0
13.297794 ABIN6390465 ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.297794 ABIN2147281 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN3028890 IF ELISA WB Rabbit Ig Fraction AA 276-304 Log in to see Polyclonal 0
4 ABIN2462186 IHC ELISA WB Rabbit Log in to see Polyclonal 0
4 ABIN2462187 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN2318209 IF IHC ELISA WB Rabbit IgG AA 435-463, Center, Glu443, Internal Region Log in to see Polyclonal 0
1 ABIN2318213 ELISA WB Rabbit IgG AA 275-304, Center, Internal Region, Pro291 Log in to see Polyclonal 0
1 ABIN1916335 ELISA WB APC Rabbit IgG AA 276-304 Log in to see Polyclonal 0
1 ABIN1916340 ELISA WB HRP Rabbit IgG AA 276-304 Log in to see Polyclonal 0
1 ABIN1916338 ELISA WB PE Rabbit IgG AA 276-304 Log in to see Polyclonal 0
1 ABIN1916337 ELISA WB FITC Rabbit IgG AA 276-304 Log in to see Polyclonal 0
1 ABIN1916336 ELISA WB Biotin Rabbit IgG AA 276-304 Log in to see Polyclonal 0
1 ABIN1916342 ELISA ICC IF IHC WB APC Rabbit IgG AA 428-455 Log in to see Polyclonal 0
1 ABIN1916341 ELISA ICC IF IHC WB Alkaline Phosphatase (AP) Rabbit IgG AA 428-455 Log in to see Polyclonal 0
1 ABIN1916344 ELISA ICC IF IHC WB FITC Rabbit IgG AA 428-455 Log in to see Polyclonal 0
1 ABIN1916346 ELISA ICC IF IHC WB HRP Rabbit IgG AA 428-455 Log in to see Polyclonal 0
1 ABIN1916345 ELISA ICC IF IHC WB PE Rabbit IgG AA 428-455 Log in to see Polyclonal 0
1 ABIN1916334 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 276-304 Log in to see Polyclonal 0
1 ABIN1916343 ELISA ICC IF IHC WB Biotin Rabbit IgG AA 428-455 Log in to see Polyclonal 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Human
(232), (56), (38), (12), (11), (11), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(125), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(16), (11), (10), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(217), (134), (124), (91), (63), (57), (41), (24), (21), (17), (13), (9), (2), (1), (1)
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Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Primate
Purification Antigen affinity purified
Immunogen The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes The stated application concentrations are suggested starting amounts. Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment The Nucleolin antibody should be aliquoted and and stored frozen at -20°C. Avoid repeat freeze-thaws.

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL ...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embed...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight ...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Western blot analysis of Nucleolin antibody and 293 cell lysate (2 ug/lane) either no...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight ...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embed...
 image for anti-Nucleolin (NCL) antibody (ABIN3032071) Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL ...