anti-Human Nucleolin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human
230
62
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Mouse
121
116
1
Clonality
Monoclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
209
131
125
88
63
58
38
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN3025715
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19 ABIN3025707 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
19 ABIN3025709 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
18.446512 ABIN153181 ChIP EM ICC IF IHC IHC (p) IM SimWes WB Rabbit Polyclonal 19
16 ABIN3025716 IHC (p) Mouse IgG 364-5-NCL-902 0
16 ABIN3025717 FACS IF IHC (p) WB Mouse IgG 364-5-NCL-902 0
16 ABIN3025708 IHC (p) Mouse IgG1 NCL-902 0
13 ABIN5584268 FACS IF IHC (p) WB Mouse IgG1 kappa NCL-902 0
13 ABIN3025710 FACS IF IHC (p) WB Mouse IgG1, kappa 364-5 0
13 ABIN3025347 FACS IF IHC (p) WB Mouse IgG1 NPC23-2 0
13 ABIN5584269 FACS IF IHC (p) WB Mouse IgG1 kappa NCL-902 0
10 ABIN5531757 IF IHC (p) WB Rabbit Ig Fraction AA 428-455 Polyclonal 0
10 ABIN5584265 IF IHC (p) WB Mouse IgG1 130CT43-8-5 0
10 ABIN3025348 IHC (p) Mouse IgG1 NPC23-2 0
10 ABIN3025711 IHC (p) Mouse IgG1, kappa 364-5 0
10 ABIN3025349 FACS IF IHC (p) WB Mouse IgG1 NPC23-2 0
10 ABIN3025712 FACS IF IHC (p) WB Mouse IgG1, kappa 364-5 0
7 ABIN202879 IHC IHC (p) WB Rabbit IgG C-Term Polyclonal 0
7 ABIN5584266 FACS IF IHC (p) WB Mouse IgG1 kappa 364-5 0
7 ABIN5584267 FACS IF IHC (p) WB Mouse IgG1 kappa 364-5 0
7 ABIN1737582 IF IHC (p) WB Rabbit AA 284-709 Polyclonal 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Human
(230), (62), (38), (12), (11), (11), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(121), (116), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Nucleolin antibody is un-conjugated
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(209), (131), (125), (88), (63), (58), (38), (24), (21), (17), (13), (9), (2), (1), (1)
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Protein G affinity chromatography
Immunogen SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
Clone 364-5-NCL-902
Isotype IgG
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes Optimal dilution of the Nucleolin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Nucleolin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025715) Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Nucleolin anti...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025715) Formalin-fixed, paraffin-embedded human colon carcinoma stained with AF488 Conjugate ...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025715) Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Nucleolin anti...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025715) Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Nucleolin a...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025715) Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025715) Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin antibody.