anti-Mouse (Murine) Polypyrimidine Tract Binding Protein 2 antibody for ELISA

Recommended Polypyrimidine Tract Binding Protein 2 Antibody (supplied by: Log in to see )

Antigen
Polypyrimidine Tract Binding Protein 2 (PTBP2) Antibodies
  • PTB
  • PTBLP
  • brPTB
  • nPTB
  • nPTB5
  • nPTB6
  • nPTB7
  • nPTB8
  • Ptb2
  • PTBP2
  • im:7153495
  • ptbp2
  • si:ch211-160l17.2
  • wu:fa08f05
  • wu:fc05d10
  • zgc:113074
  • polypyrimidine tract binding protein 2
  • polypyrimidine tract binding protein 2b
  • polypyrimidine tract binding protein 2a
  • PTBP2
  • Ptbp2
  • ptbp2b
  • ptbp2a
Epitope
Internal Region
9
6
4
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
42
19
17
5
4
4
4
3
3
3
1
1
1
Host
Goat
25
14
5
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
Application
ELISA
31
29
3
2
2
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN238583
$ 291.53
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN6264500 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN2462292 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN297348 ELISA IHC IHC (p) Goat AA 44-54 Log in to see Polyclonal 0
1 ABIN2466161 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN646977 ELISA WB Goat Log in to see 0
1 ABIN1090460 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2564785 ELISA Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN2344506 ELISA WB Goat Log in to see Polyclonal 0
1 ABIN2920972 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5872862 ELISA WB Rabbit IgG Log in to see Polyclonal 0
-6.659155 ABIN262285 ELISA Goat Log in to see Polyclonal 1

General

Antigen Polypyrimidine Tract Binding Protein 2 (PTBP2) Antibodies
Epitope Internal Region
(9), (6), (4), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(42), (19), (17), (5), (4), (4), (4), (3), (3), (3), (1), (1), (1)
Host Goat
(25), (14), (5)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (1), (1), (1)
Application ELISA
(31), (29), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Purpose PTBP2
Blocking Peptide Blocking peptide for this product available: ABIN369883
Sequence SKKFKGEDKM D
Predicted Reactivity Human, Mouse, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Recent
Immunogen Peptide with sequence C-SKKFKGEDKMD, from the internal region of the protein sequence according to NP_067013.1 .

Target Details

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Antigen
Alternative Name PTBP2 (PTBP2 Antibody Abstract)
Background PTBP2, polypyrimidine tract binding protein 2, FLJ34897, MIBP, PTB, PTBLP, brPTB, nPTB, nPTB5, nPTB6, nPTB7, nPTB8, PTB-like, neural polypyrimidine tract binding protein, splicing regulator
Gene ID 58155
NCBI Accession NP_067013
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

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Application Notes

DS WB Results: Preliminary experiments gave an approx 35 kDa band in lysates of cell line NIH3T3 after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5 kDa according to NP_067013.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References

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Background publications

Boutz, Stoilov, Li, Lin, Chawla, Ostrow, Shiue, Ares, Black: "A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neurons." in: Genes & development, Vol. 21, Issue 13, pp. 1636-52, 2007