anti-Rat (Rattus) Methionine Adenosyltransferase II, alpha antibody for Simple Western

Recommended Methionine Adenosyltransferase II, alpha Antibody (supplied by: Log in to see )

Antigen
Methionine Adenosyltransferase II, alpha (MAT2A) Antibodies
  • MATA2
  • MATII
  • SAMS2
  • Sams2
  • D630045P18Rik
  • fd12a12
  • mat2a
  • si:ch73-340n8.1
  • wu:fb95e01
  • wu:fd12a12
  • m(2)21ab
  • MGC76253
  • MGC79598
  • zgc:110847
  • methionine adenosyltransferase 2A
  • methionine adenosyltransferase II, alpha
  • methionine adenosyltransferase II, alpha a
  • methionine adenosyltransferase II, alpha L homeolog
  • methionine adenosyltransferase II, alpha b
  • MAT2A
  • Mat2a
  • mat2aa
  • mat2a.L
  • mat2a
  • mat2ab
Epitope
AA 100-200, Internal Region
16
13
9
7
6
5
2
2
1
1
Reactivity
Cow (Bovine), Human, Primate, Rat (Rattus), Zebrafish (Danio rerio)
63
12
12
3
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
49
16
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
1
1
1
Application
Simple Western (SimWes), Western Blotting (WB)
60
30
14
10
5
4
1
1
Options
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Catalog No. ABIN410051
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General

Antigen Methionine Adenosyltransferase II, alpha (MAT2A) Antibodies
Epitope AA 100-200, Internal Region
(16), (13), (9), (7), (6), (5), (2), (2), (1), (1)
Reactivity Cow (Bovine), Human, Primate, Rat (Rattus), Zebrafish (Danio rerio)
(63), (12), (12), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(49), (16), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (4), (3), (2), (2), (2), (1), (1), (1)
Application Simple Western (SimWes), Western Blotting (WB)
(60), (30), (14), (10), (5), (4), (1), (1)
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Product details

Target Details Application Details Handling Images
No Cross-Reactivity Mouse (Murine)
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of the human MAT2A protein (within residues 100-200). [Swiss-Prot# P31153]

Target Details

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Antigen
Alternative Name MAT2A (MAT2A Antibody Abstract)
Background Gene Symbol: MAT2A
Molecular Weight Theoretical MW: 43 kDa
Gene ID 4144
UniProt P31153
Pathways Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process

Application Details

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Application Notes Western Blot 2 μg/mL, Simple Western 1:10This MAT1/2A antibody is useful for Western blot, where a band is seen at approx. 43 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for MAT1/2A Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-MAT1/2A primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

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Supplier Images
Simple Western (SimWes) image for anti-Methionine Adenosyltransferase II, alpha (MAT2A) (AA 100-200), (Internal Region) antibody (ABIN410051) Simple Western: MAT1/2A Antibody [ABIN4100512] - Simple Western lane view shows a spe...
Western Blotting (WB) image for anti-Methionine Adenosyltransferase II, alpha (MAT2A) (AA 100-200), (Internal Region) antibody (ABIN410051) Western Blot: MAT1/2A Antibody [ABIN4100512] - Detection of MAT2A in HepG2 whole cell...