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Rockland Antibodies

Accelerate your research with Original Rockland Antibodies, now available at antibodies-online. These ready-to-use primary antibodies from various hosts are ideally suited for studies on apolipoproteins, apoptosis, epigenetics, and the extracellular matrix. Thanks to their outstanding affinity and specificity, Rockland Antibodies enable precise purification, detection, and quantification of your target antigens. Rely on our selection at antibodies-online to achieve reliable results and sustainably advance your scientific work. We offer you excellent conditions and exceptionally fast delivery times.

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2,770 results

COL1A2/COL1A1 antibody

Rockland 600-401-103-0.1 Reactivity: Human, Cow, Mouse, Pig, Rat ELISA, WB, DB, FLISA, FACS, FM, IHC, IP Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

IHC

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Immunofluorescence staining of extracellular and intracellular type I collagen.

IF (cc)

Reduced secretion of type I collagen by Akt inhibition and S451A overexpression.

Anti-fibrotic effect of apigenin in human HSC line (TWNT-4 cells).

The testing of multi-differentiation of rabbit TSCs in vivo.

IHC

ASH1 positively influences the expression of TIMP-1, TGF-β-1, and Collagen I.

LPA increases α-SMA and ECM production in HTM cells.

AICAR treatment reduces post-MI adverse remodeling.

Immunohistochemistry of Collagen I antibody.

IHC

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

IHC

Effect of ex vivo treatment with Pirfenidone and Nintedanib on the fibrotic phenotype of 3D-LTCs.

SIM mutant as dominant negative protein for type I collagen expression.

Transgenic VEGFA overexpression reduced bleomycin-induced lung fibrosis.

IHC

Zoledronic acid (ZA) at the maximum concentration in serum (Cmax) inhibits the transforming growth factor-β (TGF-β)-induced myofibroblast (MF) differentiation of human gingival fibroblasts (hGFs).

HJC0123 inhibits hepatic stellate cells activation and decreases endogenous ECM proteins expression.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells.

Age-related alterations in collagen expression and structure.

CCR2 mediates the specific accumulation of type I collagen positive exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury.

FACS

Oridonin suppresses α-SMA and ECM protein expression.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

IHC

CYD0692 suppresses endogenous and TGF-β induced ECM protein expression.

Immunofluorescence and SHG detection of collagens in NAFLD stage 0 fibrosis.

Inhibition of mTORC1 reduces secretion of type I collagen.

Decreased gene and protein expression of Tnmd in Scx-deficient mice.

ISH

Representative histological analysis for collagen quantification of (a) native and (b) decellularized equine lung tissue.

IHC

Expression of collagen by 5 day cultured spleen cells.

FACS

Pulmonary fibroblasts deposit elastin and collagen within Gelfoam matrices.

Inhibition of collagen prolyl hydroxylase by PDC significantly reduced collagen production in vitro and attenuated BLM-induced lung fibrosis in vivo.

IF (cc)

46 images

62 references

2 validations

Catalog No. ABIN5596819

Details

Independently Validated

DYKDDDDK Tag antibody

Rockland 600-401-383 WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

Affinity Purified Antibody to detect conjugated proteins detects both C terminal linked and N terminal linked tagged recombinant proteins by western blot.

Western Blot of Rabbit anti-FLAG antibody.

antibody to detect conjugated proteins is shown to detect as little as 3 ng of amino-terminal tagged recombinant protein by western blot.

8 images

21 references

2 validations

Catalog No. ABIN99294

Details

Independently Validated

ATM antibody (pSer1981)

Rockland 200-301-400 ATM Reactivity: Human WB, IHC, ELISA, IF, IP, FACS, FM Host: Mouse Monoclonal 10H11-E12 unconjugated

Anti-ATMpS1981 Flow Cytometry Flow Cytometry of Mouse anti-ATMpS1981 antibody.

FACS

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy Anti ATM antibody showing overlay of anti-ATM pS1981 staining.

Anti-ATM Monoclonal Antibody-Immunohistochemistry.

ATM is activated by spermidine in GM00637 cells.

ICC

12 images

126 references

Catalog No. ABIN6656104

Details

Rockland Original

APP antibody

Rockland 600-401-253 APP Reactivity: Human, Mouse WB, ELISA, IHC, IF, FM Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037)

IHC

Western Blot of Rabbit anti-Beta Amyloid antibody.

Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH.

Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C).

IHC

ELISA

Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC

Immunofluorescence (IF) image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037)

9 images

1 reference

2 validations

Catalog No. ABIN95037

Details

Independently Validated

Collagen IV antibody

Rockland 600-401-106-0.1 COL4 Reactivity: Human, Cow WB, ELISA, IHC, IP, DB, FM Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

IHC

anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids.

12 images

8 references

1 validation

Catalog No. ABIN5596835

Details

Independently Validated

COL3A1 antibody

Rockland 600-401-105-0.1 COL3A1 Reactivity: Human, Cow, Pig WB, IHC, ELISA, IP, FACS, DB, FM Host: Rabbit Polyclonal unconjugated

IHC

Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C Secondary antibody: 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: ABIN925618 for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa

anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.

IHC

Immunohistochemistry of Rabbit Anti-collagen type III antibody.

IHC

(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin.

Typical electropherogram of α (III) subunits, oligomers β/γ (III), and collagen type III degradation products, released from healthy and burned skin.

9 images

5 references

1 validation

Catalog No. ABIN5596830

Details

Independently Validated

ATM antibody (pSer1981)

Rockland 200-301-400s ATM Reactivity: Human WB, IHC, ELISA, IF, IP, FACS, FM Host: Mouse Monoclonal 10H11-E12 unconjugated

IHC

FACS

5 images

124 references

Catalog No. ABIN6657494

Details

Rockland Original

MCL-1 antibody (Internal Region)

Rockland 600-401-394 MCL1 Reactivity: Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

Inactivation of GSK3β by p38 MAPK promotes accumulation of the prosurvival factor Mcl-1.

ELISA

Rabbit anti-Mcl-1 Western Blot of Rabbit anti-Mcl-1 antibody.

The down-regulation of Bim protein occurs downstream of the activation of Erk1/2 and Kit mutant in Kit-driven leukemic cells.

S1P1 regulates expression of pro-survival protein Mcl-1.

The enhanced survival capacity of S1P1-expressing cells requires new protein synthesis and is lost upon Mcl-1 downregulation.

Activation of endogenous S1P receptors by FTY720P induces Mcl-1 to delay the onset of apoptosis in vascular ECs following growth factor withdrawal.

IF (cc)

10 images

85 references

Catalog No. ABIN6657786

Details

Rockland Original

SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) antibody

Rockland 200-401-a50 SARS-CoV N Reactivity: SARS Coronavirus (SARS-CoV), SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, IHC, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody.

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2.

mIHC

This immunofluorescence microscopy image shows localization of SARS-CoV-2 infection in human liver ductal organoids.

Immunofluorescence of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody.

Western blot using anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46-kDa band corresponding to the protein.

Western Blot of Rabbit Anti-SARS-CoV (N) Antibody tested on Caco-2 Cell Lysates before (mock), 24, and 48 hours post infection (hpi).

Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.

IHC

Immunohistochemistry of Rabbit Anti-SARS CoV Nucleocapsid Antibody.

IHC

ELISA

Immunofluorescence of Rabbit Anti-SARS CoV (N) Protein Antibody.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis.

ELISA

14 images

97 references

Catalog No. ABIN6952544

Details

Rockland Original

TERT antibody (C-Term)

Rockland 600-401-252s TERT Reactivity: Human WB, ELISA, IHC, IP, IF, FM Host: Rabbit Polyclonal unconjugated

Rabbit anti-hTERT WB Western Blot of Rabbit anti-Telomerase catalytic subunit antibody.

Anti-hTERT Antibody - Immunofluorescence Microscopy anti hTERT antibody-Immunofluorescence# anti hTERT antibody was used to stain hTERT on hTERT-over-expressing fibroblasts.

Anti-hTERT Antibody - Immunofluorescence Microscopy Immunofluorescence microscopy of Saos-2 cells transduced with a retroviral vector expressing hTERT and green fluorescent protein (GFP) from an internal ribosomal entry site (IRES).

Anti-hTERT Antibody - Immunoprecipitation/Western Blot Immunoprecipitation of hTERT protein from NIH 3T3 cell lysates.

6 images

44 references

Catalog No. ABIN6655122

Details

Rockland Original

beta Actin antibody (AA 350-375)

Rockland 600-401-886 ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-Beta Actin Antibody.

Immunohistochemistry of Rabbit Anti-Beta Actin Antibody.

Distinct functions of HOXD proteins in regulation of neuronal differentiation.

E2F6 is essential for HOXC9 induction of G1 arrest and repression of cell cycle genes.

Effects of TRPM2 inhibition and RNAi knockdown in cancerous and non-cancerous breast cell lines after chemotherapeutic treatments.

Analysis of poly(ADP-ribose)-mediated caspase-independent cell death in breast adenocarcinoma cells after TRPM2 inhibition and chemotherapeutic treatments.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

10 images

31 references

Catalog No. ABIN129657

Details

Rockland Original

TERT antibody (C-Term)

Rockland 600-401-252 TERT Reactivity: Human WB, ELISA, IHC, IP, IF, FM Host: Rabbit Polyclonal unconjugated

Sub-cellular localization of TERT and MMP-9 in SCCF2 and SCCF3.

IF (cc)

Expression of TERT, cMyc, and telomerase activity in SCCF2 and SCCF3 (A,B) TERT and cMyc gene expression in SCCF2 and SCCF3.

8 images

46 references

Catalog No. ABIN6655270

Details

Rockland Original

HIS2AV (pSer137) antibody

Rockland 600-401-914 Reactivity: Drosophila melanogaster ELISA, IHC, WB Host: Rabbit Polyclonal unconjugated

Western blot using affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head).

Systemic hormetic responses from muscle-specific DNA damage.

IF (p)

IF (cc)

WB analyses with αdH1, αγH2Av and αH4 of increasing amounts of extracts (lanes 1-3) prepared from siRNAdH1, siRNAlacZ and untreated cells.

WB analysis with αγH2Av and αtubulin at different time points after X-ray irradiation (10Gy) of siRNAdH1 and untreated cells.

DNA damage induced by dH1 depletion associates with R-loops accumulation.

IF (cc)

WB analyses with αdH1, αγH2Av and αH4 antibodies of siRNAdH1 and untreated cells sorted at G1-, S and G2/M-phase.

Immunostaining with αγH2Av (green) and αCaspase 3 (red) of wing imaginal discs from dH1-depleted his1RNAi flies upon dATRRNAi co-depletion (bottom) or not (top).

hth mutant nuclei show increased number of DNA breaks.

IF (p)

B, B´) However, hth mutant nuclei show more nuclear signal when stained with the same antibody (see arrows in B´) indicating that they have more breaks in their DNA.

IF (p)

10 images

27 references

Catalog No. ABIN129671

Details

Rockland Original

beta Catenin antibody (C-Term)

Rockland 600-401-c68 CATNB Reactivity: Human, Zebrafish (Danio rerio) WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Catenin, beta (CATNB) (C-Term) antibody (ABIN1043907)

Western Blot of Rabbit anti-Beta Catenin antibody.

Western Blot of Rabbit anti-catenin ß-1 antibody Lane 1: zebrafish embryos injected with myc tagged catenin ß 1 mRNA Lane 2: zebrafish embryos injected with myc tagged catenin ß 2 mRNA Lane 3: zebrafish embryos un-injected Primary antibody: catenin ß-1 antibody at 1:500 overnight at 4°C Secondary antibody: goat anti-rabbit HRP at 1:10,000 for 1 hour at RT Predicted/Observed size: 85.

Immunohistochemistry of Rabbit anti-beta Catenin antibody.

IHC

4 images

2 references

1 validation

Catalog No. ABIN1043906

Details

Independently Validated

JAG1 antibody (N-Term)

Rockland 200-401-698 JAG1 Reactivity: Human, Mouse WB, ELISA, IHC, IF, FM Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Jagged 1 (JAG1) (N-Term) antibody (ABIN1043767)

Western blot using Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody.

IHC

Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.

4 images

2 references

1 validation

Catalog No. ABIN129524

Details

Independently Validated

MCL-1 antibody (Internal Region)

Rockland 600-401-394s MCL1 Reactivity: Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

Characterization of clonal lymphoid lines mutant for combinations of pro-apoptotic BCL2 family proteins.

ELISA

Rabbit anti-Mcl-1 WB Western Blot of Rabbit anti-Mcl-1 antibody.

4 images

86 references

Catalog No. ABIN6657787

Details

Rockland Original

RFP antibody

Rockland 200-301-379 RFP Reactivity: Discosoma WB, ELISA, IP, FACS, FM Host: Mouse Monoclonal 8E5-G7 unconjugated

Characterization of the transgenic model and effect of 4D on the RMSAFluorescence image of a sagittal section of a 4D+ brain after a 4-day treatment with doxycycline showing RFP signal confined to the SVZ and RMS (nuclei counterstained with DAPI, blue).

9 images

16 references

Catalog No. ABIN1607680

Details

Rockland Original

PDCD4 antibody (C-Term)

Rockland 600-401-965 PDCD4 Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

H2O2 causes cytoplasmic accumulation of HuR and a loss in PDCD4 expression that is mediated by miR-21A.

Western blot using affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead).

Western Blot of Rabbit anti-PDCD antibody.

HuR regulates PDCD4 stability via miR-21A.

HuR directly binds to PDCD4 3'UTR mRNA to regulate its protein expressionA.

ERK8 phosphorylates HuR to prevent its binding to PDCD4 mRNAA.

8 images

11 references

Catalog No. ABIN129709

Details

Rockland Original

SMAD3 antibody (pSer423, pSer425)

Rockland 600-401-919 SMAD3 Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit anti-SMAD3 pS423 pS425 antibody.

affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.

IHC

Western blot using affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates.

Proximity between AcSDKP and FGFR1 inhibits the TGFβ/smad signaling pathway in HMVECs.

AcSDKP suppresses TGFβ/smad signaling and EndMT through the FGFR1/FRS2 pathway.

MAP4K4 deficiency induces TGFβ/smad signaling and EndMT via activation of integrin β1.

7 images

18 references

Catalog No. ABIN129675

Details

Rockland Original

APOA1 antibody

Rockland 600-101-196 APOA1 Reactivity: Mouse WB, IHC, ELISA, IP Host: Goat Polyclonal unconjugated

Immunofluorescence of Anti-Apolipoprotein AI.

Immunohistochemistry of Anti-Apolipoprotein AI.

IHC

8 images

10 references

Catalog No. ABIN5596808

Details

Rockland Original

Fibronectin 1 antibody (Biotin)

Rockland 600-406-117 FN1 Reactivity: Human, Mouse, Rat, Cow, Monkey WB, ELISA, IHC, IP Host: Rabbit Polyclonal Biotin

Immunohistochemistry of Rabbit Anti-Fibronectin Antibody.

IHC

Immunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negative controls (right).

IHC

4 images

1 validation

Catalog No. ABIN5596762

Details

Independently Validated

Glucagon antibody

Rockland 600-401-mv9 GCG Reactivity: Human WB, IHC, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Glucagon (GCG) antibody (ABIN7448121)

IHC

Western Blot of Rabbit Anti-Glucagon Antibody.

ELISA

4 images

1 validation

Catalog No. ABIN7448121

Details

Independently Validated

TJP1 antibody

Rockland 600-401-gu7s TJP1 Reactivity: Human WB, IHC, ELISA, IF, FACS, FM, MA Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit anti-ZO-1 antibody Western Blot of Rabbit Anti-ZO-1 Antibody.

Immunofluorescence microscopy of ZO-1 Immunofluorescence microscopy of Anti-ZO-1 in Caco-2 cells using FITC-conjugated Fluorescent anti-rabbit IgG for detection.

Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody.

Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody.

Immunohistochemistry of Rabbit anti-ZO-1 antibody Immunohistochemistry of Rabbit anti-ZO-1 antibody.

IHC

6 images

1 reference

Catalog No. ABIN6655950

Details

Rockland Original

His Tag antibody

Rockland 600-401-382 WB, ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated

A knock-in mouse line with NALCN tagged with GFP, HA, and His tags (NALCN-GFP-HA-His mice).

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot.

Western blot of Rabbit Anti-6xHIS Epitope Tag antibody.

Crude extracts prepared from Salmonella strains with the phoR-8×Myc gene expressing PhoU-His, PhoUR184A-His, PhoUR184G-His, or the empty vector (pBAD33) were detected with anti-Myc (top) and anti-His (bottom) antibodies.

$Eluted fractions prepared from strains listed above were detected with anti-Myc (top) and anti-His (bottom) antibodies after immunoprecipitation with anti-Myc antibody-coated beads.$ WB

5 images

14 references

Catalog No. ABIN100492

Details

Rockland Original

TUBA1B antibody (C-Term)

Rockland 200-301-880 TUBA1B Reactivity: Human WB, ELISA, IF, IHC, FM, MA Host: Mouse Monoclonal 17H11-F10 unconjugated

Scx is required for the expression of Fmod and Adamtsl2.

Immunofluorescence (IF) image for anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibody (ABIN964563)

Immunofluorescence of Mouse monoclonal anti-Alpha Tubulin antibody.

Immunofluorescences of Mouse monoclonal anti-Alpha Tubulin antibody.

HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 µg of lysate each.

Smad3 is required for TGF-β to regulate expression of Fmod and Adamtsl2.

TGF-β signaling regulates noncanonical pathways in the sclerotome.

AKT and p38 are not required for TGF-β-mediated regulation of fibrous tissue markers.

ERK is required for fibrous tissue marker regulation but not required to inhibit chondrogenesis.

13 images

6 references

Catalog No. ABIN964563

Details

Rockland Original

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