anti-Rat (Rattus) EGFR antibody for Western Blotting

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • LOC5564544
Epitope
AA 25-346
148
127
69
64
57
57
53
53
52
49
49
48
43
41
39
35
32
31
31
30
27
27
26
25
22
22
18
18
18
17
17
17
17
16
16
16
15
15
15
15
15
15
15
14
10
8
7
7
7
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
2488
890
718
26
25
18
17
14
12
7
2
2
2
1
1
1
1
Host
Rabbit
1512
867
84
44
12
5
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
102
83
74
65
56
54
54
32
32
28
28
28
28
28
28
28
26
25
24
24
21
21
21
21
21
21
20
18
17
17
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
1545
708
667
621
554
282
272
227
134
89
67
55
43
40
19
14
12
11
9
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN3042517
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1807668 ABIN2788648 WB Rabbit Middle Region Log in to see Polyclonal 0
3.1807668 ABIN2706075 IC IF IHC WB Rabbit pSer693 Log in to see Polyclonal 0
3.1807668 ABIN2704749 WB Rabbit C-Term, pSer1071 Log in to see Polyclonal 0
3.1807668 ABIN2704750 IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.1807668 ABIN2706077 IHC WB Rabbit Center Log in to see Polyclonal 0
3.1807668 ABIN2706076 WB Rabbit pSer678 Log in to see Polyclonal 0
3.1807668 ABIN2706078 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
3.1807668 ABIN2704752 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.1807668 ABIN2704751 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.1807668 ABIN967602 IHC (f) ELISA WB Mouse IgG1, kappa pTyr845 Log in to see 12A3 7
3.1807668 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
3.1807668 ABIN1532007 ELISA WB Rabbit IgG AA 991-1040, pSer1026 Log in to see Polyclonal 1
3.1807668 ABIN1532008 ELISA WB Rabbit IgG AA 1036-1085, pSer1071 Log in to see Polyclonal 1
3.1807668 ABIN1532602 ELISA WB Rabbit IgG AA 1031-1080 Log in to see Polyclonal 1
3.1807668 ABIN1532852 IHC ELISA WB Rabbit IgG AA 1139-1188 Log in to see Polyclonal 1
3.1807668 ABIN1533045 ELISA WB Rabbit IgG AA 1036-1085 Log in to see Polyclonal 1
3.1807668 ABIN1533046 ELISA WB Rabbit IgG AA 966-1015 Log in to see Polyclonal 1
3.1807668 ABIN1533044 ELISA WB Rabbit IgG AA 991-1040 Log in to see Polyclonal 1
3.1807668 ABIN1872454 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1807668 ABIN1532331 IF IHC ELISA WB Rabbit IgG AA 986-1035 Log in to see Polyclonal 0

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 25-346
(148), (127), (69), (64), (57), (57), (53), (53), (52), (49), (49), (48), (43), (41), (39), (35), (32), (31), (31), (30), (27), (27), (26), (25), (22), (22), (18), (18), (18), (17), (17), (17), (17), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (14), (10), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(2488), (890), (718), (26), (25), (18), (17), (14), (12), (7), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1512), (867), (84), (44), (12), (5), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(102), (83), (74), (65), (56), (54), (54), (32), (32), (28), (28), (28), (28), (28), (28), (28), (26), (25), (24), (24), (21), (21), (21), (21), (21), (21), (20), (18), (17), (17), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Western Blotting (WB)
(1545), (708), (667), (621), (554), (282), (272), (227), (134), (89), (67), (55), (43), (40), (19), (14), (12), (11), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN3042517) Images
Purpose Rabbit IgG polyclonal antibody for Epidermal growth factor receptor(EGFR) detection. Tested with WB in Human,Rat.
Brand Picoband™
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Epidermal growth factor receptor(EGFR) detection. Tested with WB in Human,Rat.
Gene Name: epidermal growth factor receptor
Protein Name: Epidermal growth factor receptor
Purification Immunogen affinity purified.
Immunogen E.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN3042517) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Synonyms: Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody|Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody|Cell growth inhibiting protein 40 antibody|Cell proliferation inducing protein 61 antibody|EGF R antibody|EGFR antibody|EGFR_HUMAN antibody|Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody|Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody|Epidermal growth factor receptor antibody|erbb 1 antibody|Erbb antibody|Erbb1 antibody|ERBB1 antibody|Errp antibody|HER1 antibody|mENA antibody|Oncogene ERBB antibody|PIG61 antibody|Proto-oncogene c-ErbB-1 antibody|Receptor tyrosine protein kinase ErbB 1 antibody|Receptor tyrosine protein kinase ErbB1 antibody|Receptor tyrosine-protein kinase ErbB-1 antibody|Urogastrone antibody|wa2 antibody|Wa5 antibody
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN3042517) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN3042517) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-EGFR antibody (ABIN3042517)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Yue, Gao, Xue, Cui, Zhao, Yuan, Yin, Inagaki, Kokudo, Tang, Qu: "Des-?-carboxyl prothrombin induces matrix metalloproteinase activity in hepatocellular carcinoma cells by involving the ERK1/2 MAPK signalling pathway." in: European journal of cancer (Oxford, England : 1990), Vol. 47, Issue 7, pp. 1115-24, 2011

Gu, Zhang, Wu, Xu, Cui, Yang, Zhao, Sun: "Differential expression of decorin, EGFR and cyclin D1 during mammary gland carcinogenesis in TA2 mice with spontaneous breast cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 6, 2010

Ge, Yu, Petitte, Zhang: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." in: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, 2009

Wang, Cheng, Cui, Zhong, Ward, Sun, Chen, Kokudo, Tang, Qu: "Des-gamma-carboxy prothrombin stimulates human vascular endothelial cell growth and migration." in: Clinical & experimental metastasis, Vol. 26, Issue 5, pp. 469-77, 2009

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN3042517) back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 25-346) antibody (ABIN3042517) Anti-EGFR Picoband antibody, All lanes: Anti-EGFR at 0.5ug/ml Lane 1: HELA Whole Cel...