anti-Dog (Canine) EPH Receptor A2 antibody for Immunocytochemistry

Recommended EPH Receptor A2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A2 (EPHA2) Antibodies
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • epha2
  • MGC89505
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • id:ibd5152
  • rtk6
  • G42
  • XE10
  • epha2-A
  • EPH receptor A2
  • Eph receptor A2
  • epoxide hydrolase
  • eph receptor A2 a
  • EPH receptor A2 L homeolog
  • EPHA2
  • Epha2
  • epha2
  • ephA2
  • epha2a
  • epha2.L
Epitope
Extracellular Domain
28
27
25
22
18
15
15
15
14
12
11
10
9
8
7
7
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine)
282
169
57
21
14
9
7
7
4
4
2
2
2
2
1
Host
Mouse
212
92
36
2
2
1
Clonality
Monoclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
19
15
14
14
13
9
8
7
7
6
6
6
6
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
251
167
98
67
54
27
17
13
8
6
5
4
2
2
2
2
2
1
1
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Catalog No. ABIN204192
$ 881.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN211915 Func ICC IP WB Mouse IgG1 Log in to see 0

General

Antigen EPH Receptor A2 (EPHA2) Antibodies
Epitope Extracellular Domain
(28), (27), (25), (22), (18), (15), (15), (15), (14), (12), (11), (10), (9), (8), (7), (7), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine)
(282), (169), (57), (21), (14), (9), (7), (7), (4), (4), (2), (2), (2), (2), (1)
Host Mouse
(212), (92), (36), (2), (2), (1)
Clonality
Monoclonal
Conjugate This EPH Receptor A2 antibody is un-conjugated
(19), (15), (14), (14), (13), (9), (8), (7), (7), (6), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
(251), (167), (98), (67), (54), (27), (17), (13), (8), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1)
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Product Details anti-EPH Receptor A2 Antibody

Target Details EPH Receptor A2 Application Details Handling Images
Specificity Recognizes human EphA2.
Purification Protein G purified
Immunogen NS0-derived rhEphA2 extracellular domain.
Isotype IgG2a

Target Details EPH Receptor A2

Product Details anti-EPH Receptor A2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPHA2 / EPH Receptor A2 (EPHA2 Antibody Abstract)
Background Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1
Gene ID 1969
UniProt P29317
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling Images back to top
Application Notes Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with 200 μL sterile PBS.
Concentration Lot specific
Buffer Lyophilized from PBS, pH 7.4, 5 % trehalose
Handling Advice Avoid frequent freeze thaws. Store undiluted.
Storage -20 °C,-80 °C
Storage Comment Store at -20°C to -70°C. Stable for 12 months from date of receipt.