anti-Human Galectin 3 antibody for Western Blotting

Recommended Galectin 3 Antibody (supplied by: Log in to see )

Antigen
Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
  • CBP 35
  • Gal-3
  • Galectin-3
  • leg3
  • galactoside-binding
  • galectin-3
  • lectin
  • soluble
  • CBP35
  • GAL3
  • GALBP
  • GALIG
  • L31
  • LGALS2
  • MAC2
  • MGC52947
  • MGC75803
  • LGALS3
  • gal-3
  • GBP
  • L-34
  • Mac-2
  • gal3
  • galectin 3
  • Galectin-3
  • avian beta-defensin 3
  • lectin, galactoside-binding, soluble, 3 L homeolog
  • lectin, galactoside-binding, soluble, 3
  • galectin 2
  • lectin, galactose binding, soluble 3
  • LGALS3
  • leg3
  • AvBD3
  • lgals3.L
  • lgals3
  • LGALS2
  • Lgals3
Epitope
AA 163-191, C-Term
41
32
21
15
14
14
13
13
12
9
9
8
6
6
6
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
385
194
98
14
3
2
2
1
1
1
1
Host
Rabbit
227
186
49
22
Clonality
Polyclonal
Conjugate
This Galectin 3 antibody is un-conjugated
39
23
21
16
15
10
9
7
5
5
4
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
360
200
132
118
98
77
47
43
26
14
11
8
6
6
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN655879
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3323684 ABIN657647 IHC (p) WB Rabbit Ig Fraction AA 167-196, C-Term Log in to see Polyclonal 4
3.3323684 ABIN152697 ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see A3A12 2
3.3323684 ABIN952435 EIA FACS WB Rabbit Ig Fraction C-Term, AA 170-199 Log in to see Polyclonal 5
3.3323684 ABIN659197 WB Mouse IgG1 AA 163-191, C-Term Log in to see 320CT4-5-2 3
3.3323684 ABIN3043399 IHC (p) WB Rabbit IgG AA 139-250 Log in to see Polyclonal
3.3323684 ABIN4899436 CyTOF FACS IHC SimWes WB Goat IgG AA 2-250 Log in to see Polyclonal 3
3.3323684 ABIN1533284 IF IHC ELISA WB Rabbit IgG AA 141-190 Log in to see Polyclonal
3.3323684 ABIN5611538 EIA IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3323684 ABIN223285 WB Rabbit IgG Log in to see Polyclonal
3.3323684 ABIN4899435 CyTOF FACS WB Mouse IgG2b AA 2-250 Log in to see 194801
3.3323684 ABIN4890614 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
3.3323684 ABIN5662023 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 1-250 Log in to see
3.3323684 ABIN3187710 ELISA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.3323684 ABIN3183706 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3323684 ABIN2706202 IC IF IHC WB Rabbit Center Log in to see Polyclonal
3.3323684 ABIN1536595 IHC (p) WB Mouse IgG1, kappa AA 163-191, C-Term Log in to see 320CT4-5-2
3.3323684 ABIN4313234 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.3323684 ABIN517489 WB Rabbit AA 1-250, full length Log in to see Polyclonal 1
3.3323684 ABIN223644 WB Rabbit IgG Log in to see Polyclonal
3.3323684 ABIN5663915 IHC WB Rabbit IgG pSer3 Log in to see Polyclonal

General

Antigen Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
Epitope AA 163-191, C-Term
(41), (32), (21), (15), (14), (14), (13), (13), (12), (9), (9), (8), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(385), (194), (98), (14), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(227), (186), (49), (22)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Galectin 3 antibody is un-conjugated
(39), (23), (21), (16), (15), (10), (9), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(360), (200), (132), (118), (98), (77), (47), (43), (26), (14), (11), (8), (6), (6), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Galectin 3 Antibody

Target Details Galectin 3 Application Details Handling References for anti-Galectin 3 antibody (ABIN655879) Images
Specificity This LGALS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human LGALS3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This LGALS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 AA from the C-terminal region of human LGALS3.
Clone RB31668
Isotype Ig Fraction

Target Details Galectin 3

Product Details anti-Galectin 3 Antibody Application Details Handling References for anti-Galectin 3 antibody (ABIN655879) Images back to top
Antigen
Alternative Name LGALS3 (LGALS3 Antibody Abstract)
Background This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.
Synonyms: Galectin-3,LGALS3,MAC2
Molecular Weight 26152 DA
Gene ID 3958
NCBI Accession NP_002297
UniProt P17931
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure
Pathways RTK Signaling

Application Details

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Handling References for anti-Galectin 3 antibody (ABIN655879) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details References for anti-Galectin 3 antibody (ABIN655879) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-Galectin 3 antibody (ABIN655879)

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details Handling Images back to top
Background publications

Salomonsson, Carlsson, Osla, Hendus-Altenburger, Kahl-Knutson, Oberg, Sundin, Nilsson, Nordberg-Karlsson, Nilsson, Karlsson, Rini, Leffler: "Mutational tuning of galectin-3 specificity and biological function." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 35079-91, 2010

Debierre-Grockiego, Niehus, Coddeville, Elass, Poirier, Weingart, Schmidt, Mazurier, Guérardel, Schwarz: "Binding of Toxoplasma gondii glycosylphosphatidylinositols to galectin-3 is required for their recognition by macrophages." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 32744-50, 2010

Mazurek, Conklin, Byrd, Raz, Bresalier: "Phosphorylation of the beta-galactoside-binding protein galectin-3 modulates binding to its ligands." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36311-5, 2000

Images

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details Handling References for anti-Galectin 3 antibody (ABIN655879) back to top
Supplier Images
Western Blotting (WB) image for anti-Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) (AA 163-191), (C-Term) antibody (ABIN655879) LGALS3 Antibody (C-term) (ABIN655879) western blot analysis in MCF-7 cell line lysate...