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|Antigen||Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This Galectin 3 antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-Galectin 3 AntibodyTarget Details Galectin 3 Application Details Handling Images
|Specificity||The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3's hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.|
|Purification||Protein A or G purified|
|Immunogen||The antibody reacts with the Galectin-3 antigen.|
|Plasmids, Primers & others|
Target Details Galectin 3Product Details anti-Galectin 3 Antibody Application Details Handling Images back to top
|Alternative Name||Galectin-3 (LGALS3 Antibody Abstract)|
|Background||Gene Symbol: LGALS3|
Application DetailsProduct Details anti-Galectin 3 Antibody Target Details Galectin 3 Handling Images back to top
|Application Notes||Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Functional, ImmunoassayThe monoclonal antibody B2C10 can be used for Western blotting, flow cytometry and immunohistology on both frozen and paraffin embedded sections. Furthermore the monoclonal antibody B2C10 is useful for immuno assays and functional studies.For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of galectin-3 to be inhibited.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details Images back to top
PBS, containing 0.1 % bovine serum albumin.
Buffer contains: No Preservative
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 4°C. Do not freeze.|