anti-Pig (Porcine) HER2 antibody for ELISA

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
pThr686
101
95
72
69
61
58
55
55
48
35
26
22
21
21
21
20
20
20
20
20
20
20
20
20
19
19
19
17
17
15
15
13
13
12
11
8
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
1386
501
324
35
33
14
12
7
5
4
4
3
3
3
2
1
1
1
Host
Rabbit
1006
523
38
16
13
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
55
47
41
21
21
14
14
13
12
12
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
720
601
334
321
309
309
269
134
111
102
91
22
21
19
8
6
6
5
5
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN576254
$ 581.17
Plus shipping costs $45.00

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pThr686
(101), (95), (72), (69), (61), (58), (55), (55), (48), (35), (26), (22), (21), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (17), (17), (15), (15), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(1386), (501), (324), (35), (33), (14), (12), (7), (5), (4), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(1006), (523), (38), (16), (13), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (55), (47), (41), (21), (21), (14), (14), (13), (12), (12), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
(720), (601), (334), (321), (309), (309), (269), (134), (111), (102), (91), (22), (21), (19), (8), (6), (6), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Specificity Recognizes human HER2/neu, otherwise known as CD340 and c-ErbB-2, phosphorylated at threonine residue (amino acid 686). Some binding to unphosphorylated HER2/neu may be seen at higher concentrations, but this may be overcome by appropriate titration. HER2/neu is a proto-oncogene expressed at the cell surface of a range of tumor cells, which is often associated with poor prognosis, and an increased risk of metastasis, and is used in conjunction with antibodies recognizing both Estrogen and Progesterone receptors, for the classification of breast cancer tumors, and to determine patient prognosis and course of treatment. Excessive HER2/neu is present in around 20 % to 25 % of invasive breast cancers, referred to as HER2-positive cancer, and can help determine whether drugs such as trastuzumab (Herceptin) or lapatinib (Tykerb), might be beneficial.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Cat, Bovine, Bat, Dog, Horse, Pig (100%) Opossum, Trichoplax (82%).
Immunogen Threonine phosphorylated peptide KIRKYTMRRLL from CD340 sequence. ( pThr686 ). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Cat, Bovine, Bat, Dog, Horse, Pig (100%), Opossum, Trichoplax (82%).
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes Approved: ELISA, IHC-Fr (1:500), WB (1:1000 - 1:5000)

Usage: Western Blot: For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10 mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Serum, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Store undiluted.
Storage 4 °C,-20 °C
Storage Comment +4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.