anti-Rat (Rattus) HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 1081-1130, pTyr1112
101
92
71
65
60
54
54
48
46
35
26
22
22
21
20
20
20
20
20
20
20
20
20
19
19
19
17
17
15
15
13
13
13
12
11
8
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1359
490
319
36
34
15
13
8
6
5
4
3
3
3
2
2
1
1
1
Host
Rabbit
992
511
32
15
12
5
2
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
56
55
41
19
19
12
12
11
10
10
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
ELISA, Western Blotting (WB)
710
603
318
314
309
304
259
133
112
102
89
21
19
18
8
6
6
5
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN1532014
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3319917 ABIN1533053 ELISA WB Rabbit IgG AA 1081-1130 Log in to see Polyclonal 2
3.3319917 ABIN1534423 IF IHC ELISA WB Rabbit IgG AA 641-690 Log in to see Polyclonal 1
3.3319917 ABIN1532623 IHC ELISA WB Rabbit IgG AA 661-710 Log in to see Polyclonal 2
3.3319917 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 2
3.3319917 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal
3.3319917 ABIN4309120 IHC IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal 1
3.3319917 ABIN2957599 IHC WB Rabbit Log in to see Polyclonal
3.3319917 ABIN2957598 IC IF IHC WB Rabbit Log in to see Polyclonal
3.3319917 ABIN1531861 ELISA IHC IP WB Rabbit IgG AA 851-900, pTyr877 Log in to see Polyclonal
3.3319917 ABIN1531860 IHC ELISA WB Rabbit IgG AA 1206-1255, pTyr1248 Log in to see Polyclonal
3.3319917 ABIN1531859 ELISA IHC IP WB Rabbit IgG AA 1191-1240, pTyr1221 Log in to see Polyclonal
3.3319917 ABIN1532891 IHC ELISA WB Rabbit IgG AA 1206-1255 Log in to see Polyclonal
3.3319917 ABIN1532892 IHC ELISA WB Rabbit IgG AA 851-900 Log in to see Polyclonal
3.3319917 ABIN1532890 IHC ELISA WB Rabbit IgG AA 1191-1240 Log in to see Polyclonal
3.3319917 ABIN126789 IF WB Mouse IgG1 pTyr1112 Log in to see 19G5
3.3319917 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal
3.3319917 ABIN4309122 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.3319917 ABIN3188001 IHC WB Mouse IgG1 Log in to see 11H9
3.3319917 ABIN2852635 IHC WB Mouse Log in to see
3.3319917 ABIN498415 IF IHC (p) WB Rabbit Log in to see Polyclonal

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1081-1130, pTyr1112
(101), (92), (71), (65), (60), (54), (54), (48), (46), (35), (26), (22), (22), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (17), (17), (15), (15), (13), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1359), (490), (319), (36), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(992), (511), (32), (15), (12), (5), (2)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (56), (55), (41), (19), (19), (12), (12), (11), (10), (10), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application ELISA, Western Blotting (WB)
(710), (603), (318), (314), (309), (304), (259), (133), (112), (102), (89), (21), (19), (18), (8), (6), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN1532014) Images
Specificity

HER2 (Phospho-Tyr1112) Antibody detects endogenous levels of HER2 only when phosphorylated at Tyr1112.

PhosphorylationH:Y1112 M:Y1113 R:Y1114

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1112.
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN1532014) Images back to top
Antigen
Alternative Name HER2 (ERBB2 Antibody Abstract)
Background

Synonyms: C-erbB-2, Epidermal growth factor receptor-related protein, ErbB2, HER2, kinase ErbB2, MLN 19, NEU, NEU proto-oncogene, NGL, p185erbB2, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell s

NCBI Gene Symbol: ERBB2

Molecular Weight 137 kDa
Gene ID 2064
OMIM 164870
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN1532014) Images back to top
Application Notes WB: 1:500~1:1000 ELISA: 1:10000
Comment

Unigene-Number: Hs.446352 (NCBI Gene Symbol: ERBB2)

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN1532014) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-HER2 antibody (ABIN1532014)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN1532014) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1081-1130), (pTyr1112) antibody (ABIN1532014) Western blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 20', usi...